An assessment of the overexpression of BMP‐2 in transfected human osteoblast cells stimulated by mineral trioxide aggregate and Biodentine

Aim To evaluate the effect of MTA and Biodentine on viability, osteogenic differentiation and BMP‐2 expression in osteogenic cells. Methodology Saos‐2 cells were used as a model of osteoblastic cells. Overexpression of BMP‐2 was induced by transfection of a CMV‐driven plasmid construct including the...

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Published in:International endodontic journal 2017-12, Vol.50 (S2), p.e9-e18
Main Authors: Rodrigues, E. M., Gomes‐Cornélio, A. L., Soares‐Costa, A., Salles, L. P., Velayutham, M., Rossa‐Junior, C., Guerreiro‐Tanomaru, J. M., Tanomaru‐Filho, M.
Format: Article
Language:English
Subjects:
Alkaline phosphatase
Alkaline Phosphatase - metabolism
Aluminum Compounds - pharmacology
bioactivity
Biocompatibility
Biodentine
Biological activity
Blotting, Western
Bone Morphogenetic Protein 2 - metabolism
bone morphogenetic protein‐2
Calcium
Calcium Compounds - pharmacology
Cell Differentiation - drug effects
Cell Survival - drug effects
Cells, Cultured
Cytotoxicity
Drug Combinations
Endodontics
Gene expression
Humans
Materials Testing
Mineralization
Mitochondria
MTA
Nodules
Osteoblasts
Osteoblasts - metabolism
Oxides - pharmacology
Real-Time Polymerase Chain Reaction
Silicates - pharmacology
Statistical analysis
Transfection
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Summary:Aim To evaluate the effect of MTA and Biodentine on viability, osteogenic differentiation and BMP‐2 expression in osteogenic cells. Methodology Saos‐2 cells were used as a model of osteoblastic cells. Overexpression of BMP‐2 was induced by transfection of a CMV‐driven plasmid construct including the human BMP‐2 coding sequence, and stably transfected cells were selected. Cell viability was assessed by the mitochondrial dehydrogenase enzymatic (MTT) assay. The bioactivity of the materials was evaluated by the alkaline phosphatase (ALP) assay and detection of calcium deposits with alizarin red staining (ARS). The gene expression of BMP‐2 and ALP was quantified with real‐time PCR. Statistical analysis was performed with analysis of variance and Bonferroni or Tukey post‐test (α = 0.05). Results Viability tests revealed that MTA and Biodentine were not cytotoxic at the higher dilution (1 : 8) to BMP‐2‐transfected cells. MTA and Biodentine exhibited the highest ALP activity when compared to the Saos‐BMP‐2‐unexposed control group (P 
ISSN:0143-2885
1365-2591
DOI:10.1111/iej.12745