Revealing Conformational Variants of Solution‐Phase Intrinsically Disordered Tau Protein at the Single‐Molecule Level

Intrinsically disordered proteins, such as tau protein, adopt a variety of conformations in solution, complicating solution‐phase structural studies. We employed an anti‐Brownian electrokinetic (ABEL) trap to prolong measurements of single tau proteins in solution. Once trapped, we recorded the fluo...

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Bibliographic Details
Published in:Angewandte Chemie 2017-12, Vol.129 (49), p.15790-15794
Main Authors: Manger, Lydia H., Foote, Alexander K., Wood, Sharla L., Holden, Michael R., Heylman, Kevin D., Margittai, Martin, Goldsmith, Randall H.
Format: Article
Language:English
Subjects:
Anisotropy
Biophysik
Chemical compounds
Chemistry
Compaction
Einzelmoleküluntersuchungen
Electrokinetics
Fluorescence
Fluoreszenzspektroskopie
Fluorophores
Mikrofluidik
Proteins
Proteinstrukturen
Tau protein
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Summary:Intrinsically disordered proteins, such as tau protein, adopt a variety of conformations in solution, complicating solution‐phase structural studies. We employed an anti‐Brownian electrokinetic (ABEL) trap to prolong measurements of single tau proteins in solution. Once trapped, we recorded the fluorescence anisotropy to investigate the diversity of conformations sampled by the single molecules. A distribution of anisotropy values obtained from trapped tau protein is conspicuously bimodal while those obtained by trapping a globular protein or individual fluorophores are not. Time‐resolved fluorescence anisotropy measurements were used to provide an explanation of the bimodal distribution as originating from a shift in the compaction of the two different families of conformations. Eine Anti‐Brownsche elektrokinetische Falle wurde verwendet, um Messungen einzelner Tau‐Proteine in Lösung zu verlängern. Die unterschiedlichen Konformationen der einzelnen Moleküle wurden durch Fluoreszenzanisotropie bestimmt. Die Verteilung der Anisotropiewerte von gefangenem Tau‐Protein ist im Gegensatz zu denen von globularen Proteinen und einzelnen Fluorophoren auffällig bimodal.
ISSN:0044-8249
1521-3757
DOI:10.1002/ange.201708242