In situ synthesis of the imine-based covalent organic framework LZU1 on the inner walls of capillaries for electrochromatographic separation of nonsteroidal drugs and amino acids

• Published in: Mikrochimica acta (1966) 2017-04, Vol.184 (4), p.1169-1176
• Main Authors: Kong, Deying, Bao, Tao, Chen, Zilin
• Format: Article
• Language: English
• Subjects: Amino acids; Aminopropyltriethoxysilane; Analytical Chemistry; Capillaries; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Chromatography; Columns (process); Covalence; Crosslinked polymers; Electrochromatography; Epitaxial growth; Epitaxy; Glutaraldehyde; Imines; Microengineering; Nanochemistry; Nanotechnology; Original Paper; Porosity; Selectivity; Separation; Silicon dioxide; Structural stability; Synthesis; Walls
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-017-2095-5

The covalent organic framework LZU1 (Lan Zhou University-1) is one of the imine-based covalent organic frameworks (COFs) that possesses attractive properties such as structural regularity, stability and good porosity. This work reports on the in-situ synthesis of COF-LZU1 on the inner walls of capillary column for open-tubular capillary electrochromatography (OT-CEC). The fused-silica capillary was first modified with 3-aminopropyltriethoxysilane and glutaraldehyde. Epitaxial growth of COF-LZU1 on the inner walls was accomplished by Schiff base reaction. The formation of COF-LZU1 was confirmed by SEM and FT-IR. The COF-LZU1 coating increases the interactions between analytes and coating, which remarkably improves the CEC separation selectivity of neutral analytes, amino acids and nonsteroidal anti-inflammatory drugs. The COF-LZU1-modified column exhibits good stability and repeatability. The precisions (relative standard deviations) for intra-day, inter-day and column-to column are