Preparation of surface imprinted core-shell particles via a metal chelating strategy: specific recognition of porcine serum albumin

• Published in: Mikrochimica acta (1966) 2016, Vol.183 (1), p.345-352
• Main Authors: Li, Qinran, Yang, Kaiguang, Li, Senwu, Liu, Lukuan, Zhang, Lihua, Liang, Zhen, Zhang, Yukui
• Format: Article
• Language: English
• Subjects: Albumin; Analytical Chemistry; Beads; Binding; Book publishing; Characterization and Evaluation of Materials; Chelation; Chemistry; Chemistry and Materials Science; Copper; Copper compounds; Core-shell particles; Ethylenediaminetetraacetic acid; Free radical polymerization; Methacrylic acid; Microengineering; Microparticles; Molecular imprinting; Myoglobins; Nanochemistry; Nanotechnology; Original Paper; Polyhydroxyethyl methacrylate; Polymerization; Protein binding; Proteins; Recognition; Serum albumin; Silica; Silicon dioxide; Strategy
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-015-1640-3

We describe the synthesis of molecularly imprinted core-shell microparticles via a metal chelating strategy that assists in the creation of selective recognition sites for albumin. Porcine serum albumin (PSA) was immobilized on silica beads via copper(II) chelation interaction. A solution containing 2-hydroxyethyl methacrylate and methacrylic acid as the monomers was mixed with the above particles, and free radical polymerization was performed at 25 °C. Copper ion and template were then removed to obtain PSA-imprinted core-shell particles (MIPs) with a typical diameter of 5 μm. The binding capacity of such MIP was 8.9 mg protein per gram of MIPs, and the adsorption equilibrium was established within