Catalytic nanocrystalline coordination polymers as an efficient peroxidase mimic for labeling and optical immunoassays

We report that nanocrystalline Prussian blue of the type Fe 4 [Fe(CN) 6 ] 3 is a powerful peroxidase mimic for use in labeling of biomolecules. The cubic nanocrystals typically have a diameter of 15 nm and are capable of catalyzing the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine in the pre...

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2016-02, Vol.183 (2), p.651-658
Main Authors: Čunderlová, Veronika, Hlaváček, Antonín, Horňáková, Veronika, Peterek, Miroslav, Němeček, Daniel, Hampl, Aleš, Eyer, Luděk, Skládal, Petr
Format: Article
Language:English
Subjects:
Albumin
Analytical Chemistry
Biomolecules
Biotin
Catalysis
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Coordination polymers
Hydrogen peroxide
Labeling
Microengineering
Nanochemistry
Nanocrystals
Nanotechnology
Original Paper
Oxidation
Peroxidase
Polymers
Proteins
Serum albumin
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Summary:We report that nanocrystalline Prussian blue of the type Fe 4 [Fe(CN) 6 ] 3 is a powerful peroxidase mimic for use in labeling of biomolecules. The cubic nanocrystals typically have a diameter of 15 nm and are capable of catalyzing the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine in the presence of H 2 O 2 to form an intensively colored product with an absorption maximum at 662 nm. The determined pseudo turnover number is ~20,000 s −1 which is the highest value reported for nanoparticles of a size comparable to common proteins. We also present a method for the biotinylation of the surface of these nanocrystals, and show their use in competitive bioaffinity based assays of biotin and human serum albumin. The limits of detection are 0.35 and 0.27 μg mL −1 , respectively. The results prove the applicability of coordination polymers for signal amplification and also their compatibility with the format of enzyme linked immunosorbent assays. Graphical abstract Nanocrystalline Prussian blue catalyzes the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine to form intensely colored products. Biotinylated cubic Prussian blue nanoparticles (biotin-NPs) facilitated the immunoassay of human serum albumin (HSA) utilizing biotinylated anti-human serum albumin antibody (biotin-IgG).
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-015-1697-z