Immobilized monolithic enzymatic reactor and its application for analysis of in‐vitro fertilization media samples

Classical proteomics approaches involve enzymatic hydrolysis of proteins (either separated by polyacrylamide gels or in solution) followed by peptide identification using LC‐MS/MS analysis. This method requires normally more than 16 h to complete. In the case of clinical analysis, it is of the utmos...

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Bibliographic Details
Published in:Electrophoresis 2017-11, Vol.38 (22-23), p.2957-2964
Main Authors: Chen, Wei‐Qiang, Obermayr, Philipp, Černigoj, Urh, Vidič, Jana, Panić‐Janković, Tanta, Mitulović, Goran
Format: Article
Language:English
Subjects:
Digestion
Gels
Immobilized enzymatic reactor
In vitro fertilization
On‐line digest
Proteins
Proteomics
Reproducibility
Separation
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Summary:Classical proteomics approaches involve enzymatic hydrolysis of proteins (either separated by polyacrylamide gels or in solution) followed by peptide identification using LC‐MS/MS analysis. This method requires normally more than 16 h to complete. In the case of clinical analysis, it is of the utmost importance to provide fast and reproducible analysis with minimal manual sample handling. Herein we report the method development for online protein digestion on immobilized monolithic enzymatic reactors (IMER) to accelerate protein digestion, reduce manual sample handling, and provide reproducibility to the digestion process in clinical laboratory. An integrated online digestion and separation method using monolithic immobilized enzymatic reactor was developed and applied to digestion and separation of in‐vitro‐fertilization media.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.201700197