Screening of variables in [beta]-xylosidase recovery using cetyl trimethyl ammonium bromide reversed micelles

β-Xylosidase recovery by micelles using cetyl trimethyl ammonium bromide (CTAB) cationic surfactant was verified under different experimental conditions. A 2^sup 5-1^ fractional factorial design with center points was employed to verify the influence of the following factors on enzyme extraction: pH...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2001-04, Vol.91-93 (1-9), p.719
Main Authors: Hasmann, Francislene-andréia, Pessoa, Adalberto, Roberto, Inês-conceição
Format: Article
Language:English
Subjects:
Ammonium
Statistical analysis
Studies
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Summary:β-Xylosidase recovery by micelles using cetyl trimethyl ammonium bromide (CTAB) cationic surfactant was verified under different experimental conditions. A 2^sup 5-1^ fractional factorial design with center points was employed to verify the influence of the following factors on enzyme extraction: pH (x ^sub 1^), CTAB concentration (x ^sub 2^), electrical conductivity (x ^sub 3^), hexanol concentration (x ^sub 4^), and butanol concentration (x ^sub 5^). Statistical analysis of the results shows that of the fivevariables studied only hexanol and electrical conductivity did not have significant effects on the recovery of β-xylosidase. The other factors had significant effects in increasing order: (x ^sub 1^)>(x ^sub 2^)>(x ^sub 5^). The model predicts a recovery value of about 45%, which is similar to that obtained experimentally (43.5%).[PUBLICATION ABSTRACT]
ISSN:0273-2289
1559-0291
DOI:10.1385/ABAB:91-93:1-9:719