Development of linkage map and mapping of QTLs for oil content and yield attributes in linseed (Linum usitatissimum L.)

• Published in: Euphytica 2017-11, Vol.213 (11), p.1-11, Article 258
• Main Authors: Chandrawati, Yadav, Hemant Kumar
• Format: Article
• Language: English
• Subjects: Agronomy; Biomedical and Life Sciences; Biotechnology; Crops; Fatty acids; Gene polymorphism; Genetic aspects; Genetic variability; Genotype & phenotype; Genotypes; Life Sciences; Linum usitatissimum; Mapping; Markers; Normal distribution; Oilseeds; Plant breeding; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Polymorphism; Povidone; Quantitative genetics; Quantitative trait loci; Seeds
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1007/s10681-017-2050-x

Linseed ( Linum usitatissimum L.) is an important oilseed as well as stem fiber crop and rich source of omega-3 fatty acid. The present study aims to develop linkage map based on Indian genotypes and utilize it for mapping QTLs for important agronomic traits. Two diverse parental genotypes (KL-213 and RKY-14) of linseed showed wide range of variability for oil content and yield attributes. These parental genotypes also showed reasonable level of SSR polymorphism (~ 9.0%). The mapping population showed normal distribution of phenotypic traits. One hundred forty-six SSR markers were mapped on 15 linkage groups with marker density ranging from 3 to 18 markers per linkage group at average distance of 14.2 cM. A total of 11 QTLs were identified for six quantitative traits. Three QTLs for capsules/plant, 2 QTLs each for plant height, seeds/capsule and oil content and 1 QTL each for branches/plant and seed weight/plant were detected. Phenotypic variability explained by these QTLs varied from 1 to 15.23%. This study provides framework linkage map of linseed using Indian genotypes, which needs to be enriched further for future application in marker assisted breeding of linseed.