Inheritance of Transgenes in Transgenic Tall Fescue (Festuca arundinacea Schreb.)

Tall fescue (Festuca arundinacea Schreb.) is the most important forage species worldwide of the Festuca genus. Single genotype-derived embryogenic suspension cultures were established from tall fescue cultivar Kentucky-31, and were used as target cells for biolistic transformation. A chimeric hygrom...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 2003-05, Vol.39 (3), p.277-282
Main Authors: Wang, Z. Y., Bell, J., Y. X. Ge, Lehmann, D.
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Biotechnology
Biotechnology/Genetic Transformation/Functional Genomics
DNA
Embryonic cells
Forage
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic engineering applications
Genetic inheritance
Genetic technics
Genetics and breeding of economic plants
Genomics
Methods. Procedures. Technologies
Plant breeding: fundamental aspects and methodology
Plants
Plasmids
Polymerase chain reaction
Transgenes
Transgenic animals and transgenic plants
Transgenic plants
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Summary:Tall fescue (Festuca arundinacea Schreb.) is the most important forage species worldwide of the Festuca genus. Single genotype-derived embryogenic suspension cultures were established from tall fescue cultivar Kentucky-31, and were used as target cells for biolistic transformation. A chimeric hygromycin phosphotransferase gene (hph) was used as the selectable marker, and a chimeric β-glucuronidase (gusA) gene was co-transformed with hph. Transgenic plants were recovered after microprojectile bombardment of suspension cells and subsequent selection in the presence of a high concentration of hygromycin. Fertile transgenic plants were obtained after vernalization under field conditions. T1 and T2 progenies were obtained after reciprocal crosses between transgenic and untransformed control plants. PCR and Southern hybridization analyses revealed a 1:1 segregation ratio for both transgenes in the T1 and T2 generations. Southern hybridization patterns were identical for T0, T1, and T2 plants. The results demonstrated for the first time the stable meiotic transmission of transgenes following Mendelian rules in transgenic tall fescue.
ISSN:1054-5476
1475-2689
DOI:10.1079/IVP2002392