2004 SIVB CONGRESS SYMPOSIUM PROCEEDING: BIOTECHNOLOGICAL PROGRESS IN DEALING WITH DUTCH ELM DISEASE

Applications of biotechnological tools, including genetic modification aimed at combating Dutch elm disease, are described. In vitro shoot cultures of Ulmus procera (English elm) SR4, U. glabra, U. americana, and U. parvifolia have been established and used as source material in genetic transfer exp...

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Published in:In vitro cellular & developmental biology. Plant 2005-07, Vol.41 (4), p.364-367
Main Authors: GARTLAND, KEVAN M. A., McHUGH, ANGELA T., CROW, ROBERT M., GARG, AMIT, GARTLAND, JILL S.
Format: Article
Language:English
Subjects:
Agrobacterium
Biotechnology
Ceratocystis ulmi
disease prevention
disease resistance
Epidemiology
forest trees
Fungal infections
fungal resistance
genetic engineering
genetic markers
genetic modification
genetic resistance
genetic transformation
Genetic vectors
green fluorescent protein
literature reviews
micropropagation
neomycin phosphotransferase II
ornamental trees
Pathogens
Plant diseases
plant pathogenic fungi
Plants
plasmid vectors
Reporter genes
Symposium Proceedings
tissue culture
Transgenic plants
tree diseases
Ulmus
β-glucuronidase
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Summary:Applications of biotechnological tools, including genetic modification aimed at combating Dutch elm disease, are described. In vitro shoot cultures of Ulmus procera (English elm) SR4, U. glabra, U. americana, and U. parvifolia have been established and used as source material in genetic transfer experiments. Biolistic transformation of U. procera leaf material with cauliflower mosaic virus 35S-promoted constructs resulted in transient gusA (β-glucuronidase) expression. Subsequently, regenerant U. procera have been obtained following transformation of stem pieces with wild-type tumor-inducing or root-inducing plasmid-harboring Agrobacterium strains. Genetically modified elms expressing gusA, gfp (green fluorescent protein), and nptII (neomycin phosphotransferase II) genes have been produced using disarmed vectors. Regenerant English elms have been produced following transformation with anti-fungal genes, transferred to soil, and are currently being tested for their ability to resist the Dutch elm disease fungus Ophiostoma novo-ulmi. Future prospects for fighting this fungal wilt using biotechnological tools are discussed.
ISSN:1054-5476
1475-2689
DOI:10.1079/IVP2005646