Ontogenetic changes in spot configuration (numbers, circularity, size and asymmetry) and lateral line in Neurergus microspilotus (Caudata: Salamandridae)

• Published in: Acta zoologica (Stockholm) 2018-01, Vol.99 (1), p.9-19
• Main Authors: Vaissi, Somaye, Parto, Paria, Sharifi, Mozafar
• Format: Article
• Language: English
• Subjects: adult; Adults; Aquatic reptiles; Asymmetry; Chromatophores; Circularity; Coloration; Colour; Configurations; Larvae; Lateral line; melanophore‐free region; Metamorphosis; Neurergus microspilotus; Ontogeny; postmetamorph; Receptors; Skin; spot ontogeny; Spots
• ISSN: 0001-7272; 1463-6395
• DOI: 10.1111/azo.12187

Coloration in three of four species of the genus Neurergus including N. microspilotus is characterized by the presence of yellow spots on a dark skin, but there is no available information about changes in spot configuration, speed of development and degree of association between melanophore‐free region and the lateral line. In this study, spot numbers, spot circularity, spot size and spot asymmetry were studied during larval to adult growth in N. microspilotus during July 2012 to June 2015. The mean numbers of spots increased during the late larval stage till postmetamorphic period from 13.33 ± 3.77 to 22.53 ± 4.09 and reached 42.62 ± 4.06 in adults. At the same time, the extent of spots gradually decreased in size from 5.80 ± 1.00 to 3.57 ± 0.97 mm2 and reached 3.55 ± 1.42 mm2 in adults, but the spot circularity increased from 0.48 ± 0.23 to 0.78 ± 0.49 and reached 0.80 ± 0.15 in adults. In adults, the numbers, circularity, size and asymmetry of spots remain stable with little but non‐significant changes during the study period. Histological study shows that formation of a melanophore‐free region correlates with the development of the lateral line receptors. This study demonstrates that the effects of lateral line on chromatophores persist through middle larval stages but diminish as metamorphosis completes.