Sildenafil citrate concentrations not affecting oxidative phosphorylation depress H₂O₂ generation by rat heart mitochondria

• Published in: Molecular and cellular biochemistry 2008-02, Vol.309 (1-2), p.77-85
• Main Authors: Fernandes, Maria A. S, Marques, Ricardo J. F, Vicente, Joaquim A. F, Santos, Maria S, Monteiro, Pedro, Moreno, António J. M, Custódio, José B. A
• Format: Article
• Language: English
• Subjects: Acids; Adenosine diphosphate; Animals; Biochemistry; Bioenergetics; Biomedical and Life Sciences; Calcium - pharmacology; Cardiology; Cell Respiration - drug effects; Heart; Hydrogen peroxide; Hydrogen Peroxide - metabolism; Life Sciences; Male; Medical Biochemistry; Membrane Potential, Mitochondrial - drug effects; Mitochondria; Mitochondria, Heart - drug effects; Mitochondria, Heart - enzymology; Mitochondria, Heart - metabolism; Mitochondrial Membranes - drug effects; Mitochondrial Membranes - metabolism; Oncology; Oxidation; Oxidative Phosphorylation - drug effects; Oxidative Stress - drug effects; Permeability; Permeability - drug effects; Piperazines - chemistry; Piperazines - pharmacology; Prescription drugs; Purines - chemistry; Purines - pharmacology; Rats; Rats, Wistar; Respiration; Rodents; Sildenafil Citrate; Studies; Sulfones - chemistry; Sulfones - pharmacology; Superoxide Dismutase - metabolism; Superoxides - metabolism
• ISSN: 0300-8177; 1573-4919
• DOI: 10.1007/s11010-007-9645-9

Sildenafil citrate (Viagra) is a potent and specific inhibitor of cyclic guanosine monophosphate (cGMP)-specific phosphodiesterase type 5 (PDE5), which exhibits cardioprotective action against ischemia/reperfusion injury in intact and isolated heart. The mechanism of its cardioprotective action is not completely understood, but some results suggested that sildenafil exerts cardioprotection through the opening of mitochondrial ATP-sensitive K⁺ channels (mitoKATP). However, the impact of sildenafil citrate per se on isolated heart mitochondrial function is unknown. The goal of this study was to investigate the influence of the compound on mitochondrial function (bioenergetics, Ca²⁺-induced mitochondrial permeability transition, and hydrogen peroxide (H₂O₂) generation) in an attempt to correlate its known actions with effects on heart mitochondria. It was observed that sildenafil citrate concentrations of up to 50 μM did not significantly affect glutamate/malate-supported respiration in states 2, 3, 4, oligomycin-inhibited state 3, and uncoupled respiration. The respiratory control ratio (RCR), the ADP to oxygen ratio (ADP/O), the transmembrane potential (ΔΨ), the phosphorylation rate, and the membrane permeability to H⁺, K⁺ and Ca²⁺ were not affected either. However, sildenafil citrate decreased H₂O₂ generation by mitochondria respiring glutamate/malate, and also decreased the formation of superoxide radical (O ₂ [round bullet, filled]- ) generated in a hypoxantine/xantine oxidase system. It was concluded that sildenafil citrate concentrations of up to 50 μM do not affect either rat heart mitochondrial bioenergetics or Ca²⁺-induced mitochondrial permeability transition, but it depresses H₂O₂ generation by acting as a superoxide dismutase (SOD)-mimetic. By preventing reactive oxygen species (ROS) generation, sildenafil citrate may preserve heart mitochondrial function.