Evaluation of antioxidant activity of hesperidin and its protective effect on H₂O₂ induced oxidative damage on pBR322 DNA and RBC cellular membrane

The present work was carried out to evaluate the antioxidant activity of hesperidin and to study its protective effect on H₂O₂ induced oxidative damage on pBR322 DNA and RBC cellular membrane. The in vitro assays were performed with different concentrations (2, 4, 6, 8, and 10 μg/ml, which were equi...

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Bibliographic Details
Published in:Molecular and cellular biochemistry 2009-03, Vol.323 (1-2), p.21-29
Main Authors: Kalpana, K. B, Srinivasan, M, Menon, Venugopal P
Format: Article
Language:English
Subjects:
Antioxidants
Antioxidants - chemistry
Antioxidants - pharmacology
Biochemistry
Biomedical and Life Sciences
Cardiology
Cellular biology
Deoxyribonucleic acid
DNA
DNA - chemistry
DNA - drug effects
DNA Damage
Erythrocyte Membrane - drug effects
Erythrocyte Membrane - metabolism
Free radicals
Free Radicals - metabolism
Hesperidin - chemistry
Hesperidin - pharmacology
Humans
Hydrogen peroxide
Hydrogen Peroxide - pharmacology
Life Sciences
Lipid Peroxidation
Medical Biochemistry
Membranes
Molecular Structure
Oncology
Oxidants - pharmacology
Oxidation
Oxidative Stress - drug effects
Reactive Oxygen Species - metabolism
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Summary:The present work was carried out to evaluate the antioxidant activity of hesperidin and to study its protective effect on H₂O₂ induced oxidative damage on pBR322 DNA and RBC cellular membrane. The in vitro assays were performed with different concentrations (2, 4, 6, 8, and 10 μg/ml, which were equivalent to 3.27, 6.55, 9.83, 13.10, and 16.38 μM) of hesperidin and the results clearly indicate that hesperidin at 10 μg/ml exhibited radical scavenging activity greater than that of standards like ascorbic acid and trolox. The protective effect of hesperidin on pBR322 DNA and RBC cellular membrane on treatment with different concentrations of H₂O₂ shows that hesperidin at 2.5 mM converts the open circular form (oc) of pBR322 DNA that is an indication of damage to super coiled (ccc) form and at 10 μg/ml it prevents membrane damage. Thus, our result proves hesperidin to be a valuable antioxidant that protects pBR322 DNA and RBC cellular membrane from free radical induced oxidative damage.
ISSN:0300-8177
1573-4919
DOI:10.1007/s11010-008-9960-9