Evaluation of Genetic Diversity, Population Structure, and Relationship Between Legendary Vechur Cattle and Crossbred Cattle of Kerala State, India

The legendary Vechur cattle of Kerala, described as a very short breed, and the crossbred (CB) Sunandini cattle population exhibited great phenotypic variation; hence, the present study attempted to analyze the genetic diversity existing between them. A set of 14 polymorphic microsatellites were cho...

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Bibliographic Details
Published in:Animal biotechnology 2018-01, Vol.29 (1), p.50-58
Main Authors: Radhika, G., Aravindakshan, T. V., Jinty, S., Ramya, K.
Format: Article
Language:English
Subjects:
Animal populations
Bottleneck
Bovidae
Cattle
cattle - native and crossbred
Deoxyribonucleic acid
DNA
Fluorescence
Genetic diversity
Heterozygosity
Inbreeding
Microsatellites
Phenotypic variations
Population
Population genetics
Population structure
Populations
Primers
Structural analysis
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Summary:The legendary Vechur cattle of Kerala, described as a very short breed, and the crossbred (CB) Sunandini cattle population exhibited great phenotypic variation; hence, the present study attempted to analyze the genetic diversity existing between them. A set of 14 polymorphic microsatellites were chosen from FAO-ISAG panel and amplified from genomic DNA isolated from blood samples of 30 Vechur and 64 unrelated crossbred cattle, using fluorescent labeled primers. Both populations revealed high genetic diversity as evidenced from high observed number of alleles, Polymorphic Information Content and expected heterozygosity. Observed heterozygosity was lesser (0.699) than expected (0.752) in Vechur population which was further supported by positive F IS value of 0.1149, indicating slight level of inbreeding in Vechur population. Overall, F ST value was 0.065, which means genetic differentiation between crossbred and Vechur population was 6.5%, indicating that the crossbred cattle must have differentiated into a definite population that is different from the indigenous Vechur cows. Structure analysis indicated that the two populations showed distinct differences, with two underlying clusters. The present study supports the separation between Taurine and Zebu cattle and throws light onto the genetic diversity and relationship between native Vechur and crossbred cattle populations in Kerala state.
ISSN:1049-5398
1532-2378
DOI:10.1080/10495398.2017.1297719