Effects of dietary Biogen and sodium butyrate on hematological parameters, immune response, and histological characteristics of Nile tilapia (Oreochromis niloticus) fingerlings

• Published in: Aquaculture international 2018-02, Vol.26 (1), p.139-150
• Main Authors: El-Sayed Ali, Tamer, El-Sayed, Abdelfattah Mohamed, Eissa, Mohamed Abdel-Razek, Hanafi, Hebatollah Moustafa
• Format: Article
• Language: English
• Subjects: Alanine; Biomedical and Life Sciences; Blood cells; Body weight; Control; Cytoplasm; Defence mechanisms; Diet; Feed additives; Fingerlings; Fish; Food additives; Freshwater & Marine Ecology; Freshwater fishes; Hematology; Immune response; Immunity; Life Sciences; Liver; Lymphocytes; Nucleus; Oreochromis niloticus; Parameters; Ponds; Proteins; Serum; Sodium; Tilapia; Zoology
• ISSN: 0967-6120; 1573-143X
• DOI: 10.1007/s10499-017-0205-3

The present study was carried out to evaluate the use of Biogen and sodium butyrate (SB) as feed additives in the diet of Nile tilapia ( Oreochromis niloticus ) fingerlings, in two parallel experiments. Biogen was incorporated in isonitrogenous (35% crude protein) and isocaloric (19 MJ kg −1 ) diets at four levels (0.0 (control), 0.5, 1.0, and 2.0%), while SB was included at five levels (0.0 (control), 0.5, 1.0, 2.0, or 3.0%). The diets were fed to fingerling Nile tilapia (10.5 ± 0.5 g) at a daily rate of 4% of their body weight, three times a day, for 60 days. Except the lymphocytes and monocyte numbers in fish fed SB, hematological parameters, including red blood cell (RBC) hematocrit (Ht), hemoglobin (Hb), and white blood cells (WBC) were not significantly (P  > 0.05) affected by dietary Biogen and SB. The lymphocytes number in Nile tilapia fed on SB increased with increasing SB up to 2% level, and decreased afterwards. Monocyte numbers showed irregular patterns. The activities of serum enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) of fish fed diets containing Biogen or SB were not significantly affected by dietary treatments ( P  > 0.05). No structural differences of tilapia liver were detected between all Biogen treatments and sodium butyrate concentrations up to 1% with control. At concentrations of 2 and 3% sodium butyrate, liver steatosis increased leading to shrinked acentric nuclei. At a concentration of 2% Biogen, some glomerulus cells had fading cytoplasm. Concerning fish fed SB diets, the structure of kidney was the same as in control except at concentration of 3% SB, where the septum between cells disappeared. No changes in gill structure were noticed at all concentrations of Biogen and SB.