Rapid and Sensitive Determination of L-carnitine and Acetyl-L-carnitine in Liquid Milk Samples with Capillary Zone Electrophoresis Using Indirect UV Detection

The purpose of this study was to establish a new capillary zone electrophoresis method with indirect UV detection for determining the concentration of L-carnitine and acetyl-L-carnitine in liquid milk samples. Orthogonal experimental design was applied to arrange and optimize the experimental condit...

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Bibliographic Details
Published in:Food analytical methods 2018, Vol.11 (1), p.170-177
Main Authors: Kong, Yu, Yang, Guifang, Chen, Shumeng, Hou, Zhanwu, Du, Xiaomin, Li, Hua, Kong, Linghong
Format: Article
Language:English
Subjects:
Acetyl-L-carnitine
Analytical Chemistry
Capillary electrophoresis
Capillary zone
Carnitine
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Cow's milk
Design optimization
Electrophoresis
Experimental design
Food Science
Melamine
Microbiology
Migration
Milk
Ultraviolet radiation
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Summary:The purpose of this study was to establish a new capillary zone electrophoresis method with indirect UV detection for determining the concentration of L-carnitine and acetyl-L-carnitine in liquid milk samples. Orthogonal experimental design was applied to arrange and optimize the experimental conditions. Under selected conditions (using melamine as the additive), both L-carnitine and acetyl-L-carnitine could be detected in 6 min, with satisfied reproducibilities (RSD% of migration times  0.995), recoveries (>90%), and relative low LODs (3.0 and 5.0 μmol/L for L-carnitine and acetyl-L-carnitine, respectively). The proposed method was successfully applied to the assay of nine brands of bovine milk samples and shows potential for analyzing L-carnitine and acetyl-L-carnitine in other biosamples.
ISSN:1936-9751
1936-976X
DOI:10.1007/s12161-017-0987-y