Complete nucleotide sequence of Japanese flounder (Paralichthys olivaceus) mitochondrial genome: structural properties and cue for resolving teleostean relationships

We cloned and sequenced the complete mitochondrial genome of Japanese flounder (Paralichthys olivaceus). A circular 17,090 bp mitochondrial genome from the flounder contains 37 structural genes as in other vertebrates so far reported. This is the first report of the complete mitochondrial sequence f...

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Bibliographic Details
Published in:The Journal of heredity 2000-07, Vol.91 (4), p.271
Main Authors: Saitoh, K, Hayashizaki, K, Yokoyama, Y, Asahida, T, Toyohara, H, Yamashita, Y
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Cloning, Molecular
Codon
DNA, Circular
DNA, Complementary
DNA, Mitochondrial
Fish
Flounder - classification
Flounder - genetics
Genes
Genes, Overlapping
Genetics
Genomics
Japan
Molecular Sequence Data
Mutagenesis, Insertional
Phylogeny
Replication Origin
Sequence Analysis, DNA - methods
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Summary:We cloned and sequenced the complete mitochondrial genome of Japanese flounder (Paralichthys olivaceus). A circular 17,090 bp mitochondrial genome from the flounder contains 37 structural genes as in other vertebrates so far reported. This is the first report of the complete mitochondrial sequence from a higher teleostean fish (Acanthopterygii). The organization including gene order is quite similar to that of other teleostean fishes as well as placental mammals. The putative control region of the Japanese flounder mitochondrial genome contains a length variable region of about a 74 bp tandem repeat cluster. As a preliminary study we adopted the maximum likelihood and neighbor-joining inference methods to examine phylogenetic relationships among teleostean and related fishes. Comparisons of amino acid sequences of protein-coding genes and nucleotide sequences of tRNA genes resolved some middle to deep branches among some teleostean fishes. The flounder mitochondrial genome does not show an indication of evolutionary rate difference among teleosts leading to difficulty in phylogenetic analyses, and our data is useful for future evolutionary studies dealing with higher teleostean fishes.
ISSN:0022-1503
1465-7333
DOI:10.1093/jhered/91.4.271