Targeting LC3 and Beclin-1 autophagy genes suppresses proliferation, survival, migration and invasion by inhibition of Cyclin-D1 and uPAR/Integrin β1/ Src signaling in triple negative breast cancer cells

Autophagy is a catabolic process for degrading dysfunctional proteins and organelles, and closely associated with cancer cell survival under therapeutic, metabolic stress, hypoxia, starvation and lack of growth factors, contributing to resistance to therapies. However, the role of autophagy in breas...

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Bibliographic Details
Published in:Journal of cancer research and clinical oncology 2018-03, Vol.144 (3), p.415-430
Main Authors: Hamurcu, Zuhal, Delibaşı, Nesrin, Geçene, Seda, Şener, Elif Funda, Dönmez-Altuntaş, Hamiyet, Özkul, Yusuf, Canatan, Halit, Ozpolat, Bulent
Format: Article
Language:English
Subjects:
Apoptosis
Autophagy
Breast cancer
Cancer Research
Cell growth
Cell migration
Cell proliferation
Cell survival
Cyclin D1
Growth factors
Hematology
Hypoxia
Internal Medicine
Medicine
Medicine & Public Health
Metastases
Metastasis
Oncology
Organelles
Original Article – Cancer Research
Phagocytosis
Poly(ADP-ribose) polymerase
Starvation
Therapeutic targets
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Summary:Autophagy is a catabolic process for degrading dysfunctional proteins and organelles, and closely associated with cancer cell survival under therapeutic, metabolic stress, hypoxia, starvation and lack of growth factors, contributing to resistance to therapies. However, the role of autophagy in breast cancer cells is not well understood. In the present study, we investigated the role of autophagy in highly aggressive and metastatic triple negative breast cancer (TNBC) and non-metastatic breast cancer cells and demonstrated that the knockdown of autophagy-related genes (LC3 and Beclin-1) inhibited autophagy and significantly suppressed cell proliferation, colony formation, migration/invasion and induced apoptosis in MDA-MB-231 and BT-549 TNBC cells. Knockdown of LC3 and Beclin-1 led to inhibition of multiple proto-oncogenic signaling pathways, including cyclin D1, uPAR/integrin-β1/Src, and PARP1. In conclusion, our study suggests that LC3 and Beclin-1 are required for cell proliferation, survival, migration and invasion, and may contribute to tumor growth and progression of highly aggressive and metastatic TNBC cells and therapeutic targeting of autophagy genes may be a potential therapeutic strategy for TNBC in breast cancer.
ISSN:0171-5216
1432-1335
DOI:10.1007/s00432-017-2557-5