Effect of equal daily doses achieved by different power densities of low-level laser therapy at 635 nm on open skin wound healing in normal and corticosteroid-treated rats

Optimal parameters of low-level laser therapy (LLLT) for wound healing are still discussed. Hence, our study was aimed to compare effects of different power densities of LLLT at 635 nm in rats. Four, round, full-thickness, skin wounds were made on the backs of 48 rats that were divided into two grou...

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Published in:Lasers in medical science 2009-07, Vol.24 (4), p.539-547
Main Authors: Gál, Peter, Mokrý, Michal, Vidinský, Boris, Kilík, Róbert, Depta, Filip, Harakaľová, Magdaléna, Longauer, František, Mozeš, Štefan, Sabo, Ján
Format: Article
Language:English
Subjects:
Animals
Dentistry
Glucocorticoids - therapeutic use
Granulation Tissue - pathology
Lasers
Lasers, Semiconductor - therapeutic use
Leukocytes - pathology
Low-Level Light Therapy - methods
Macrophages - pathology
Male
Medicine
Medicine & Public Health
Methylprednisolone - therapeutic use
Optical Devices
Optics
Original Article
Photonics
Quantum Optics
Rats
Rats, Sprague-Dawley
Rodents
Skin - drug effects
Skin - injuries
Skin - pathology
Skin - radiation effects
Time Factors
Wound healing
Wound Healing - drug effects
Wound Healing - radiation effects
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Summary:Optimal parameters of low-level laser therapy (LLLT) for wound healing are still discussed. Hence, our study was aimed to compare effects of different power densities of LLLT at 635 nm in rats. Four, round, full-thickness, skin wounds were made on the backs of 48 rats that were divided into two groups (non-steroid laser-treated and steroid laser-treated). Three wounds were stimulated daily with a diode laser (daily dose 5 J/cm 2 ) each with different power density (1 mW/cm 2 , 5 mW/cm 2 , and 15 mW/cm 2 ), whereas the fourth wound served as a control. Two days, 6 days, and 14 days after surgery, eight animals from each group were killed and samples were removed for histological evaluation. In the non-steroid laser-treated rats, significant acceleration of epithelization and collagen synthesis 2 days and 6 days after surgery was observed in stimulated wounds. In steroid laser-treated rats, 2 days and 14 days after surgery, a decreased leucocyte/macrophage ratio and a reduction in the area of granulation tissue were recorded, respectively. In conclusion, LLLT, by the method we used, improved wound healing in the non-steroid laser-treated rats, but it was useless after corticosteroid treatment.
ISSN:0268-8921
1435-604X
DOI:10.1007/s10103-008-0604-9