Phylogeny of Ten Kenyan Plectranthus Species in the Coleus Clade Inferred from Leaf Micromorphology, Rbcl and MatK Genes

Plectranthus species are difficult to taxonomically delimit due to lack of clear-cut morphological synapomorphies. This study is aimed at bringing insights into classification of ten Plectranthus species in the Coleus clade by using leaf micromorphology and molecular data. Stomatal counts and observ...

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Bibliographic Details
Published in:Journal of botany 2018-01, Vol.2017, p.1-16
Main Authors: Musila, Fredrick M., Lukhoba, Catherine W., Nguta, Joseph M., Dossaji, Saifuddin F.
Format: Article
Language:English
Subjects:
Biology
Cluster analysis
Clusters
Coleus
Deoxyribonucleic acid
DNA
Evolution
Flavonoids
Gene sequencing
Genes
Herbal medicine
Lamiaceae
Leaves
Morphology
Phylogenetics
Phylogeny
Plectranthus aegyptiacus
Plectranthus amboinicus
Plectranthus barbatus
Plectranthus caninus
Plectranthus edulis
Plectranthus lanuginosus
Plectranthus montanus
Plectranthus ornatus
Plectranthus otostegioides
Plectranthus pseudomarrubioides
RbcL gene
Solenostemon
Species
Species classification
Stomata
Studies
Taxonomy
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Summary:Plectranthus species are difficult to taxonomically delimit due to lack of clear-cut morphological synapomorphies. This study is aimed at bringing insights into classification of ten Plectranthus species in the Coleus clade by using leaf micromorphology and molecular data. Stomatal counts and observation of microtome leaf sections generated leaf micromorphology data, while molecular data was obtained from sequencing MatK and Rbcl genes from each species. Phylogeny based on the MatK and Rbcl gene sequences clustered four species P. caninus, P. otostegioides, P. barbatus, and P. lanuginosus together (Clusters A and D, respectively), while P. pseudomarrubioides, P. ornatus, and P. aegyptiacus were grouped together into Clusters B and E, respectively, and P. montanus and P. amboinicus were grouped together (Cluster C). A dendrogram was generated through a cluster analysis of the leaf micromorphological characters grouped together, P. caninus, P. ornatus, P. otostegioides, P. montanus, and P. pseudomarrubioides (Cluster F). The dendrogram also grouped together P. aegyptiacus, P. amboinicus, P. edulis, P. barbatus, and P. lanuginosus (Cluster G). The present study has grouped the ten studied Plectranthus species using molecular and leaf micromorphology characters into phylogenies, which are supported by previous studies, and proved that these characters can aid in plant identification and phylogenetic studies.
ISSN:2090-0120
2090-0139
DOI:10.1155/2017/4369029