Electrospun boronic acid-containing polymer membranes as fluorescent sensors for bacteria detection

In this work, a boronic acid copolymer, poly(4-vinylphenylboronic acid-co-2-(dimethylamino)ethyl methacrylate-co-n-butyl methacrylate) (pVDB) was designed for the rapid detection of bacteria based on reversible boronate ester formation with the diol-rich saccharide moieties found on bacterial membra...

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Bibliographic Details
Published in:Reactive & functional polymers 2017-12, Vol.121, p.23-31
Main Authors: Quirós, Jennifer, Amaral, Adérito J.R., Pasparakis, George, Williams, Gareth R., Rosal, Roberto
Format: Article
Language:English
Subjects:
Bacteria
Bacterial biofilms
Biofilms
Biosensor
Biosensors
Boronic acid copolymers
Chemical synthesis
E coli
Electrospinning
Electrospun membranes
Fluorescence
Free radical polymerization
Membranes
Nanofibers
Nanofibres
Polyacrylonitrile
Polymerization
Studies
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Summary:In this work, a boronic acid copolymer, poly(4-vinylphenylboronic acid-co-2-(dimethylamino)ethyl methacrylate-co-n-butyl methacrylate) (pVDB) was designed for the rapid detection of bacteria based on reversible boronate ester formation with the diol-rich saccharide moieties found on bacterial membranes. Electrospun nanofibre membranes were prepared from a blend of polyacrylonitrile (PAN) and pVDB, which was synthesized by free radical polymerization. The pVDB@PAN membranes were used as fluorescent bacterial biosensors, displaying a distinct emission at 538nm when in contact with bacteria cells. The fluorescence intensity showed a maximum intensity after 24h of contact with Staphyloccocus aureus or Escherichia coli, and this intensity increased proportionally with the pVDB content in the electrospun membranes. When in contact with Pseudomona putida, the membranes became non-responsive within 8h due to the rapid formation of a bacterial biofilm. This fouling phenomena blocked the membrane surface for fluorescence readings. The pVDB@PAN bionsensor allowed for the rapid detection of the early stages of bacterial colonization well before biofilm formation, which could be advantageous for the early identification of pathogenic bacteria and prevention of their spreading.
ISSN:1381-5148
1873-166X
DOI:10.1016/j.reactfunctpolym.2017.10.007