Catalase C-262T Gene Variant in Patients with Bronchial Asthma

• Published in: Acta Facultatis Medicae Naissensis 2017-09, Vol.34 (3), p.205-212
• Main Authors: Despotović, Milena, Stoimenov, Tatjana Jevtović, Stanković, Ivana, Bašić, Jelena, Đorđević, Branka, Pavlović, Dušica
• Format: Article
• Language: English
• Subjects: Asthma; bronchial asthma; catalase; Oxidative stress; Pathogenesis; SNP
• ISSN: 2217-2521; 0351-6083; 2217-2521
• DOI: 10.1515/afmnai-2017-0022

Bronchial asthma (BA) is a chronic inflammatory disease of the airways in the pathogenesis of which oxidative stress has a very important role. Single nucleotide polymorphisms (SNPs) in catalase gene may result in decreased antioxidative defense capacity, and thus a higher risk for BA development. Since oxidative stress has an important role in the pathogenesis of BA and catalase has a key role in antioxidant defense, the aim of this study was to examine the association of CAT C-262T polymorphism with BA in Serbian patients with BA. A total of 170 subjects (79 patients with BA and 91 controls) were screened for CAT C-262T SNP using PCR-RFLP method. The analysis of genotype distribution did not show statistically significant differences between BA patients and controls (p > 0.05). Moreover, no differences were detected when comparison was performed based on dominant or recessive model. The distribution of CAT-262C and CAT-262T alleles did not show differences between patients and healthy controls (p = 0.715; OR = 1.091; 95% CI = 0.684–1.741). Further analysis of genotype and allele distributions, based on stratification by sex, did not show significant differences between BA patients and controls (p > 0.05). This is the first study that examined CAT C-262T (rs1001179) SNP in Serbian patients with BA. The results obtained in this study showed that biallelic SNP at the position-262 in the catalase gene is not associated with BA in the Serbian population.