Evaluation of the interaction between proliferation, oxidant–antioxidant status, Wnt pathway, and apoptosis in zebrafish embryos exposed to silver nanoparticles used in textile industry

Antimicrobial textile products are developing rapidly as an important part of functional textiles. Silver nanoparticles (AgNPs) are nanotechnology products with antimicrobial properties. However, exposure to nanoparticles in daily life is an important issue for public health, still being updated. Ai...

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Bibliographic Details
Published in:Journal of biochemical and molecular toxicology 2018-01, Vol.32 (1), p.n/a
Main Authors: Eryılmaz, Oğuz, Ateş, Perihan Seda, Ünal, İsmail, Üstündağ, Ünsal Veli, Bay, Sadık, Alturfan, Ahmet Ata, Yiğitbaşı, Türkan, Emekli‐Alturfan, Ebru, Akalın, Mehmet
Format: Article
Language:English
Subjects:
Acridine orange
Antiinfectives and antibacterials
Antioxidants
Apoptosis
Catenin
Danio rerio
Embryos
Exposure
Glutathione
Glutathione transferase
Lipid peroxidation
Nanoparticles
Nanotechnology
oxidant–antioxidant status
Peroxidation
Polymerase chain reaction
Proliferating cell nuclear antigen
Public health
Silver
silver nanoparticles
Spectrophotometry
Staining
Superoxide dismutase
Textile industry
Textiles
Toxicity
Wnt pathway
Wnt protein
Zebrafish
zebrafish embryo
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Summary:Antimicrobial textile products are developing rapidly as an important part of functional textiles. Silver nanoparticles (AgNPs) are nanotechnology products with antimicrobial properties. However, exposure to nanoparticles in daily life is an important issue for public health, still being updated. Aim was to evaluate the effects of AgNPs on the development of zebrafish embryos focusing on Wnt pathway, proliferation, oxidant–antioxidant status, and apoptosis. The expressions of ccnd1 and gsk3β were determined by RT‐PCR, whereas β‐catenin and proliferative cell antigen (PCNA) expressions were determined immunohistochemically. Lipid peroxidation, superoxide dismutase, and glutathione‐S‐transferase activities were determined spectrophotometrically. Apoptosis was determined using acridine orange staining. Oxidant status, apoptosis, immunohistochemical PCNA, and β catenin staining increased, whereas ccnd1 and antioxidant enzyme activities decreased in AgNPs‐exposed embryos in a dose‐dependent manner. Our results indicate the interaction of possible mechanisms that may be responsible for the toxic effects of AgNPs in zebrafish embryos.
ISSN:1095-6670
1099-0461
DOI:10.1002/jbt.22015