Response surface optimisation of germination conditions to improve the accumulation of bioactive compounds and the antioxidant activity in quinoa

Summary Germination has been proposed as an economic approach to improve the content of bioactive compounds in pseudocereals. In this work, response surface methodology (RSM) was employed to investigate the impact of germination conditions on the phytochemical content and antioxidant activity of qui...

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Bibliographic Details
Published in:International journal of food science & technology 2018-02, Vol.53 (2), p.516-524
Main Authors: Paucar‐Menacho, Luz María, Martínez‐Villaluenga, Cristina, Dueñas, Montserrat, Frias, Juana, Peñas, Elena
Format: Article
Language:English
Subjects:
Antioxidant activity
Antioxidants
Bioactive compounds
Biological activity
Flavonoids
Germination
Health promotion
Kaempferol
Phenolic compounds
Phenols
Prediction models
Quercetin
Quinoa
Response surface methodology
Seeds
γ-Aminobutyric acid
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Summary:Summary Germination has been proposed as an economic approach to improve the content of bioactive compounds in pseudocereals. In this work, response surface methodology (RSM) was employed to investigate the impact of germination conditions on the phytochemical content and antioxidant activity of quinoa. The use of desirability methodology showed that the optimum conditions to maximise the content of total phenolic content (TPC) and antioxidant activity in sprouted quinoa were 20 °C for 42 h. Sprouts produced under these conditions exhibited increases of 80% and 30% in TPC and antioxidant activity, respectively, compared to un‐germinated seeds, and contained high γ‐aminobutyric acid (GABA) concentration. The nonsignificant lack‐of‐fit and high determination coefficients obtained confirmed the suitability of the predictive models developed for TPC and antioxidant activity, whilst the one obtained for GABA was not significant (R2 
ISSN:0950-5423
1365-2621
DOI:10.1111/ijfs.13623