A Simple Method for Monitoring Eight N-Nitrosamines in Beef Jerkys by Gas Chromatography–Tandem Mass Spectrometry with One-Step Treatment Coupled to Active Carbon Solid-Phase Extraction

• Published in: Food analytical methods 2018-03, Vol.11 (3), p.933-938
• Main Authors: Wang, Zongyi, Zhai, Mengting, Xia, Xiaonan, Yang, Man, Han, Tao, Huang, Manqing
• Format: Article
• Language: English
• Subjects: Activated carbon; Analytical Chemistry; Barium; Barium hydroxide; Beef; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Chromatography; Diethylnitrosamine; Environmental monitoring; Food Science; Gas chromatography; Ionization; Ions; Mass spectrometry; Mass spectroscopy; Microbiology; N-Nitrosodimethylamine; N-Nitrosodipropylamine; N-Nitrosomorpholine; N-nitrosopiperidine; N-Nitrosopyrrolidine; Nitrosamines; Quantitation; Scientific imaging; Spectroscopy
• ISSN: 1936-9751; 1936-976X
• DOI: 10.1007/s12161-017-1067-z

A simple method was developed for monitoring eight N -nitrosamines ( N -nitrosodimethylamine, N -nitrosomethylethylamine, N -nitrosodiethylamine, N -nitrosodipropylamine, N -nitrosomorpholine, N -nitrosopiperidine, N -nitrosopyrrolidine, and N -nitrosodibutylamine) in beef jerkys by gas chromatography–tandem mass spectrometry with one-step treatment coupled to active carbon solid-phase extraction. Sample was first treated with barium hydroxide solution, and then the sample solution is cleaned and concentrated using a Sep-Pak AC-2 cartridge. Chromatographic separation was achieved on a DB-1701 capillary column, and mass spectrometry was performed with electron-impact ionization and multiple reaction monitoring. Good linear ranges were obtained for eight analytes in the range of 1–200 ng mL −1 with the coefficient ( R 2 ) higher than 0.999. Limit of detection and limit of quantitation were in ranges of 0.05–0.10 and 0.10–0.30 ng g −1 , respectively; at 0.3, 3.0, and 10 ng g −1 spiking levels, the recoveries were 70.1–121.5% with the relative standard deviations at 3.4–17.8%. Comparing with the traditional methods, the merits of the new method are simple, relative procedure and environment-friendly, easy to use, and suitable for routine screening or analyzing samples in batch.