Chromatographic immunoassay based detection of human toxoplasmosis in District Mardan, Khyber Pakhtunkhawa, Pakistan

This study was carried out to determine the sero-prevalence of human Toxoplasmosis in district Mardan, Pakistan. A total of 600 human blood samples were collected by random sampling method. Blood samples were analyzed for Toxoplasma gondii infection and anti-T. gondii IgM and IgG antibodies by using...

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Bibliographic Details
Published in:Pure and applied biology 2017-12, Vol.6 (4), p.1297-1305
Main Author: Shah, Mudassir
Format: Article
Language:English
Subjects:
Age
Age groups
Agglutination
Antibodies
Blood
Chromatography
Females
Fetuses
Gender differences
Health care
Hygiene
Immunoassay
Immunoglobulin G
Immunoglobulin M
Immunoglobulins
Infections
Latex
Latex agglutination
Males
Medicine
Parasites
Parasitic diseases
Population
Protozoa
Random sampling
Risk analysis
Risk factors
Rural areas
Sediment pollution
Soil contamination
Soil pollution
Statistical analysis
Statistical methods
Toxoplasma gondii
Toxoplasmosis
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Summary:This study was carried out to determine the sero-prevalence of human Toxoplasmosis in district Mardan, Pakistan. A total of 600 human blood samples were collected by random sampling method. Blood samples were analyzed for Toxoplasma gondii infection and anti-T. gondii IgM and IgG antibodies by using Latex agglutination test (LAT) and Chromatographic technique, respectively. Out of 600 individuals, 105 (17.5%) were sero-positive for T. gondii. Males showed slightly higher (18.4%) prevalence as compared to female (16%), but statistically the difference was non-significant (X2= 0.394, P = 0.5302). Sero-prevalence was high (29.41%) in age group 31- 45 years and lowest (10%) in age group 0.05) of sero-positivity was observed between rural (18.06%) and urban (15.56%) areas. Seropositivity for IgG was high (24%) in age group 31-45. IgM anti-body showed highest seropositivity (14%) in age group 61-75. Of various risk factors, a significant association (P = 0.0001) was found between sero-positivity and no hand washing after contact with animal, soil or other contaminated objects. However, there was no significant relation between sero-positivity of T. gondii and educational status of subjects (p>0.05).
ISSN:2304-2478
2304-2478
DOI:10.19045/bspab.2017.600138