Ames and random amplified polymorphic DNA tests for the validation of the mutagenic and/or genotoxic potential of the drinking water disinfection by-products chloroform and bromoform

Chloroform and Bromoform are two abundant trihalomethanes found in Algerian drinking water. The investigation of the mutagenic hazard of these disinfection by-products was studied by Ames test as prokaryotic bioassay to show their mutagenic effects. For this, Salmonella typhimurium TA98 and TA100 st...

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Bibliographic Details
Published in:Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering Toxic/hazardous substances & environmental engineering, 2018-01, Vol.53 (2), p.154-159
Main Authors: Khallef, Messaouda, Cenkci, Süleyman, Akyil, Dilek, Özkara, Arzu, Konuk, Muhsin, Benouareth, Djamel eddine
Format: Article
Language:English
Subjects:
Allium cepa
Ames test
Amplification
Bacteria
Bioassays
Byproducts
Chloroform
Deoxyribonucleic acid
Disinfection
Distilled water
DNA
DNA damage
Drinking water
Genetic testing
Genotoxicity
Metabolic activation
Metabolic rate
Mutation
polymerase chain
Polymerase chain reaction
Random amplified polymorphic DNA
random amplified polymorphic DNA test
Roots
Salmonella
Salmonella Typhimurium
Strains (organisms)
Trihalomethanes
Water treatment
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Summary:Chloroform and Bromoform are two abundant trihalomethanes found in Algerian drinking water. The investigation of the mutagenic hazard of these disinfection by-products was studied by Ames test as prokaryotic bioassay to show their mutagenic effects. For this, Salmonella typhimurium TA98 and TA100 strains were employed. Both chloroform and bromoform showed a direct mutagenic effect since the number of revertant colonies gradually increase in dose-dependent manner with all concentrations tested with the two bacterial strains and these were both in the absence and presence of S9 metabolic activation. The genotoxic hazard was also studied by random amplified polymorphic DNA test on the root cells of Allium cepa as eukaryotic bioassay. DNA extracted from the roots of the onion were incubated at different concentrations of chloroform and bromoform and then amplified by polymerase chain reaction. This was based on demonstrating a major effect of disappearance of bands compared to roots incubated in the negative control (distilled water). The results showed that these two compounds affected genomic DNA by breaks although by mutations.
ISSN:1093-4529
1532-4117
DOI:10.1080/10934529.2017.1383134