Sampling of Sugarcane Leaves in Field Experiments to Determine the Activity of Nitrate Reductase

Nitrogen (N) assimilation depends on nitrate reductase activity (NRA). Leaf sampling methods in field experimentation with sugarcane and analytical procedures are essential for the determination of the maximum activity (MA) of the enzyme. Thus, in five tests, it was possible to determine which perio...

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Bibliographic Details
Published in:Communications in Soil Science and Plant Analysis 2018-01, Vol.49 (1), p.76-87
Main Authors: Santos, Renato Lemos dos, Freire, Fernando José, Oliveira, Emídio Cantídio Almeida de, Barbosa, José de Arruda, Moura, Maria José Alves de, Lopes, Nayara Rose da Conceição, Costa, Larissa Grasiela de Arruda Ferreira, Guedes, Victor Hugo de Farias, Oliveira, Diego Moura de Andrade, Santos, Monalisa Barbosa da Costa
Format: Article
Language:English
Subjects:
Collection
Diagnostic leaves
enzyme activity
Enzymes
Evaluation
Experimentation
Field tests
Incubation period
leave sampling
Leaves
Nitrate reductase
Nitrates
Nitrogen
nitrogen absorption
nitrogen assimilation
Plant tissues
Procedures
Saccharum officinarum
Sampling
Sampling methods
Sugarcane
Tissue
Vacuum
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Summary:Nitrogen (N) assimilation depends on nitrate reductase activity (NRA). Leaf sampling methods in field experimentation with sugarcane and analytical procedures are essential for the determination of the maximum activity (MA) of the enzyme. Thus, in five tests, it was possible to determine which period of the crop cycle should be used for leaf collection, the most adequate leaf for collection, the ideal number of leaves to be collected, the period of the day in which the enzyme has MA and which analytical procedures can be optimized. The evaluation of NRA in sugarcane should occur between 95 and 100 days after planting (DAP) and can be performed in any green leaf. In field experiments, it is recommended the collection of 15 leaves, which must occur between 12:00 and 12:30 h, with later incubation of the leaf tissues for 430 min, without vacuum application.
ISSN:0010-3624
1532-2416
1532-4133
DOI:10.1080/00103624.2017.1421648