Isolation and identification of Enterobacteriaceae from traditional Serbian cheese and their physiological characteristics

• Published in: Journal of food safety 2018-02, Vol.38 (1), p.n/a
• Main Authors: Mladenović, Katarina G., Muruzović, Mirjana Ž., Žugić Petrović, Tanja, Stefanović, Olgica D., Čomić, Ljiljana R.
• Format: Article
• Language: English
• Subjects: Acidity; Agglutination; Antibiotics; Bacteria; Biochemical characteristics; Biochemistry; Cheese; Chloramphenicol; Coliforms; Dairy products; E coli; Enterobacter; Enterobacteriaceae; Escherichia; Food safety; Households; Klebsiella; Lactic acid; Lactic acid bacteria; Latex; Latex agglutination; Microflora; Moisture content; Organoleptic properties; Physical characteristics; Physical properties; Proteolysis; Serratia; Sodium chloride; Species; Streptomycin; Water content
• ISSN: 0149-6085; 1745-4565
• DOI: 10.1111/jfs.12387

In this paper, the presence of enterobacteria in autochthonous cheese from Southeast Serbia (Sokobanja), made in the traditional way, was investigated. The samples from different households, was taken during the summer and the autumn. Chemical and physical characteristics of samples (water content, fat content, acidity, pH, and sodium chloride content) were examined. In the analyzed samples of cheese, four different genera of family Enterobacteriaceae (Klebsiella [65%], Escherichia [20%], Serratia [10%], and Enterobacter [5%]) were identified. The members of genus Klebsiella were Klebsiella oxytoca, Klebsiella pneumoniae, and Klebsiella ornithinolytica, while in the genus Escherichia only one species, Escherichia coli was identified. Besides, two species of the genus Serratia, Serratia odorifera and Serratia marcescens biogp 1, and one species of the genus Enterobacter, Enterobacter gergoviae, were identified. Biochemical characteristics, proteolytic and lipolytic activity, sensitivity to streptomycin, chloramphenicol, and tetracycline were, also, determined. According to the rapid latex agglutination test, of the four E. coli isolates, two isolates belong to serotype E. coli O157. Practical applications The results of this study contribute to better understanding of unexplored microflora of cheese from Sokobanja. The isolated genera were Klebsiella sp., Escherichia sp., Enterobacter sp., and Serratia sp. with twenty identified species. It is well known that bacteria from family Enterobacteriaceae could affect organoleptic properties of cheese. Bacteria isolated from cheese from Sokobanja mostly did not manifest proteolytic and lipolytic activity. The most number of isolates were susceptible to tested antibiotics. Further researches may contribute to better understanding of interaction between enterobacteria and lactic acid bacteria in autochthonous cheeses.