Nucleotide and nucleoside involvement in immunomodulation in experimental Chagas disease

• Published in: Molecular and cellular biochemistry 2018-10, Vol.447 (1-2), p.203-208
• Main Authors: do Carmo, Guilherme M., de Sá, Mariângela F., Baldissera, Matheus D., Grando, Thirssa H., Mendes, Ricardo E., Cardoso, Valesca V., Casali, Emerson A., Moritz, Cesar Eduardo J., Monteiro, Silvia G., Da Silva, Aleksandro S.
• Format: Article
• Language: English
• Subjects: Adenosine; Adenosine diphosphate; Adenosine monophosphate; Adenosine triphosphate; Alkaloids; AMP; Animal experimentation; Animals; ATP; ATP (Adenosine triphosphate); ATPases; Biochemistry; Biomedical and Life Sciences; Cardiology; Chagas Cardiomyopathy - immunology; Chagas Cardiomyopathy - parasitology; Chagas Cardiomyopathy - pathology; Chagas disease; Disease; Disease control; Disease Models, Animal; Female; Health aspects; High-performance liquid chromatography; Immune system; Immunology; Immunomodulation; Infection; Inflammation; Life Sciences; Liquid chromatography; Medical Biochemistry; Mice; Muscle proteins; Nucleosides; Oncology; Protozoa; Purine Nucleosides - immunology; Purine Nucleotides - immunology; Purines; Trypanosoma cruzi - immunology; Trypomastigotes; Uric acid; Vector-borne diseases
• ISSN: 0300-8177; 1573-4919
• DOI: 10.1007/s11010-018-3304-1

The aim of this study was to evaluate whether Trypanosma cruzi infections cause alterations in the levels of seric purines, which could contribute to host immunomodulation. Twelve mice were divided into two groups identified as control (uninfected) and infected ( T. cruzi ) groups. The influence of the disease on seric purine levels was verified on day 20 post-infection (PI) by HPLC. Infected mice had circulating trypomastigotes during the experiment, as well as amastigote forms in the heart associated with inflammatory infiltrates. Increases on adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine (ADO), inosine (INO), and uric acid (URIC) levels were observed in the infected animals, while the adenosine monophosphate (AMP) and xanthine (XAN) levels were reduced compared with mice of the control group, indicating a possible impairment on the purinergic system, and consequently, on the immune system during the clinical course of the disease. In summary, the T. cruzi infection alters the seric purine levels, and consequently, modulates the immune system.