Ligand density-dependent influence of arginine-glycine- aspartate functionalized gold nanoparticles on osteogenic and adipogenic differentiation of mesenchymal stem cells

Extracellular matrix （ECM） plays a very important role in regulating cell function and fate. It is highly desirable to fabricate biomimetic models to investigate the role of ECM in stem cell differentiation. In this study, arginine- glycine--aspartate （RGD）-modified gold nanoparticles （Au NPs） with...

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Bibliographic Details
Published in:Nano research 2018-03, Vol.11 (3), p.1247-1261
Main Authors: Li, Jingchao, Chen, Ying, Kawazoe, Naoki, Chen, Guoping
Format: Article
Language:English
Subjects:
Alkaline phosphatase
Arginine
Atomic/Molecular Structure and Spectra
Biocompatibility
Biomaterials
Biomedical engineering
Biomedical materials
Biomedicine
Biomimetics
Biotechnology
Calcium
Cell differentiation
Chemistry and Materials Science
Condensed Matter Physics
Cytoskeleton
Density
Differentiation (biology)
Extracellular matrix
Gene expression
Glycine
Gold
Ligands
Materials Science
Mesenchymal stem cells
Mesenchyme
Nanoparticles
Nanotechnology
Research Article
Stem cells
Studies
Tissue engineering
密度依赖
干细胞
ar
金
标记基因
RGD
微型环境
细胞骨架
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Summary:Extracellular matrix （ECM） plays a very important role in regulating cell function and fate. It is highly desirable to fabricate biomimetic models to investigate the role of ECM in stem cell differentiation. In this study, arginine- glycine--aspartate （RGD）-modified gold nanoparticles （Au NPs） with tunable surface ligand density were prepared to mimic the ECM microenvironment. Their effect on osteogenic and adipogenic differentiation of human mesenchymal stem cells （MSCs） was investigated. The biomimetic Au NPs were taken up by MSCs in a ligand density-dependent manner. The biomimetic NPs with a high RGD density had an inhibitive effect on the alkaline phosphatase （ALP） activity, calcium deposition, and osteogenic marker gene expression of MSCs. Their effect on oil droplet formation and adipogenic marker gene expression was negative when RGD density was low, while their effect was promotive when RGD density was high. The biomimetic Au NPs regulated the osteogenic and adipogenic differentiation of MSCs mainly through affecting the focal adhesion and cytoskeleton. This study highlights the roles of biomimetic NPs on stem cell differentiation that could provide a meaningful strategy in fabricating functional biomaterials for tissue engineering and biomedical applications.
ISSN:1998-0124
1998-0000
DOI:10.1007/s12274-017-1738-5