Production of Humic Acid by a Bacillus megaterium Strain Using Vinasse

Humic acid can be produced by a Bacillus megaterium strain, AS019, using vinasse (sugarcane mill wastewater) at an optimal concentration of 12 °Bx. Using single factor and orthogonal experiments, the optimal culture conditions were determined: optimum inoculum size, 15%; initial pH, 6.0; culture tim...

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Bibliographic Details
Published in:Sugar tech : an international journal of sugar crops & related industries 2018-04, Vol.20 (2), p.163-167
Main Authors: Li, Nan, Deng, Zhi-Nian, Wei, Yuan-Wen, Cao, Hui-Qing, Li, Yang-Rui
Format: Article
Language:English
Subjects:
Acid production
Acids
Agricultural biotechnology
Agriculture
Amino acids
Bacillus megaterium
Biomedical and Life Sciences
Functional groups
Humic acid
Humic acids
Infrared analysis
Inoculum
Life Sciences
Optimization
Research Article
Spectral analysis
Sugarcane
Vinasse
Wastewater
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Summary:Humic acid can be produced by a Bacillus megaterium strain, AS019, using vinasse (sugarcane mill wastewater) at an optimal concentration of 12 °Bx. Using single factor and orthogonal experiments, the optimal culture conditions were determined: optimum inoculum size, 15%; initial pH, 6.0; culture time, 8 days; rotation speed, 180 rpm; and temperature, 28 °C. Under the optimal conditions, the humic acid production rate for the strain was 49.50 g/L. The results of characterization and infrared spectral analysis revealed that the produced humic acid contained a variety of reactive functional groups.
ISSN:0972-1525
0974-0740
0972-1525
DOI:10.1007/s12355-017-0554-2