Effects of Influenza Derived Peptide on CD8 T Cell Responses to MHC Class I-Restricted Human Telomerase Reverse Transcriptase (hTERT)-Derived Peptide

Tumor cells in breast cancer are immunogenic and express proteins that can induce immune responses. One important antigen is human telomerase reverse transcriptase (hTERT). The main aim of this study was to use an epitope from hTERT accompanied by an epitope derived from influenza virus restricted t...

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Bibliographic Details
Published in:International journal of peptide research and therapeutics 2019-06, Vol.25 (2), p.413-418
Main Authors: Navashenaq, Jamshid Gholizadeh, Shabgah, Arezoo Gowhari, Hashemi, Esmat Alsadat, Seyedzadeh, Mir Hadi, Shokri, Fazel, Razavi, Seyed Alireza, Kardar, Gholam Ali
Format: Article
Language:English
Subjects:
Animal Anatomy
Biochemistry
Biomedical and Life Sciences
Breast cancer
CD8 antigen
Cell proliferation
Cytotoxicity
Enzyme-linked immunosorbent assay
Epitopes
Histocompatibility antigen HLA
Histology
Immune response
Immunogenicity
Influenza
Leukocytes (mononuclear)
Life Sciences
Lymphocytes T
Major histocompatibility complex
Molecular Medicine
Morphology
Peptides
Peripheral blood mononuclear cells
Pharmaceutical Sciences/Technology
Pharmacology/Toxicology
Polymer Sciences
Proteins
RNA-directed DNA polymerase
Telomerase
Telomerase reverse transcriptase
Tumor cells
Viruses
γ-Interferon
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Summary:Tumor cells in breast cancer are immunogenic and express proteins that can induce immune responses. One important antigen is human telomerase reverse transcriptase (hTERT). The main aim of this study was to use an epitope from hTERT accompanied by an epitope derived from influenza virus restricted to HLA-A2, a common Class I HLA in Iran, to induce T cells against tumoral cells. The epitopes were analyzed in IEDB and EpiMHC databases for the strength of binding to HLA-A2 and immunogenicity, respectively. Peripheral blood mononuclear cells (PBMCs) from 11 HLA-A2-positive breast cancer patients were isolated and then were harvested and co-cultured with MCF-7 cells that were previously trans-loaded with synthesized peptides. PBMC proliferation, interferon-γ (IFN-γ) secretion, and activated T cell cytotoxicity were analyzed by MTT, ELISA, and LDH cytotoxicity assays, respectively. Proliferation of PBMCs incubated with the tumoral peptide was significantly greater than that of controls ( P  
ISSN:1573-3149
1573-3904
DOI:10.1007/s10989-018-9683-z