One-Pot Multi-Enzymatic Production of Purine Derivatives with Application in Pharmaceutical and Food Industry

• Published in: Catalysts 2018-01, Vol.8 (1), p.9
• Main Authors: Acosta, Javier, del Arco, Jon, Martinez-Pascual, Sara, Clemente-Suárez, Vicente, Fernández-Lucas, Jesús
• Format: Article
• Language: English
• Subjects: Catalysts; Cellular communication; Chemical reactions; Derivatives; Food processing industry; Hypoxanthine; Lactobacilli; Nucleosides; Organic compounds; Production methods; Substrates
• ISSN: 2073-4344; 2073-4344
• DOI: 10.3390/catal8010009

Biocatalysis reproduce nature’s synthetic strategies in order to synthesize different organic compounds. Natural metabolic pathways usually involve complex networks to support cellular growth and survival. In this regard, multi-enzymatic systems are valuable tools for the production of a wide variety of organic compounds. Methods: The production of different purine nucleosides and nucleoside-5′-monophosphates has been performed for first time, catalyzed by the sequential action of 2′-deoxyribosyltransferase from Lactobacillus delbrueckii (LdNDT) and hypoxanthine-guanine-xanthine phosphoribosyltransferase from Thermus themophilus HB8 (TtHGXPRT). Results: The biochemical characterization of LdNDT reveals that the enzyme is active and stable in a broad range of pH, temperature, and ionic strength. Substrate specificity studies showed a high promiscuity in the recognition of purine analogues. Finally, the enzymatic production of different purine derivatives was performed to evaluate the efficiency of multi-enzymatic system LdNDT/TtHGXPRT. Conclusions: The production of different therapeutic purine nucleosides was efficiently catalyzed by LdNDT/TtHGXPRT. In addition, the resulting by-products were converted to IMP and GMP. Taking all of these features, this bioprocess entails an efficient, sustainable, and economical alternative to chemical synthetic methods.