Analysis of internal (n-1)mer deletion sequences in synthetic oligodeoxyribonucleotides by hybridizationto an immobilized probe array

The purity of a drug substance can influence its toxicity and potency, so impurities must be specifically determined. In the case of synthetic oligodeoxyribonucleotide drugs, however, product complexity makes complete impurity speciation difficult. The goal of the present work was to develop a new a...

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Bibliographic Details
Published in:Nucleic acids research 1999-01, Vol.27 (2), p.389
Main Authors: Chen, Danhua, Yan, Zhengming, Cole, Douglas L, Srivatsa, G Susan
Format: Article
Language:English
Online Access:Get full text
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Summary:The purity of a drug substance can influence its toxicity and potency, so impurities must be specifically determined. In the case of synthetic oligodeoxyribonucleotide drugs, however, product complexity makes complete impurity speciation difficult. The goal of the present work was to develop a new analytical method for speciation of individual internal (n-1)mer impurities arising from formal nucleotide deletion in synthetic oligodeoxyribonucleotides. A complete series of oligodeoxyribonucleotide probes were designed, each complementary to an (n-1)mer deletion sequence of the drug in question. Glass plates were used as a solid support for individually immobilizing the entire probe array. The total mixture of internal (n-1) length impurities was isolated from a synthetic oligodeoxyribonucleotide by PAGE and labeled with
ISSN:0305-1048
1362-4962