Enhancer activity of HS2 of the human [beta]-LCR is modulated by distance from the key nucleosome

A class of curved DNA appears universally in eukaryotic genomic DNA at an average distance of ~680 bp and shows nucleosome positioning activity by having high affinity for histone core particles in an orientation- and position-dependent manner. Here, we report that the enhancer activity at DNase I h...

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Bibliographic Details
Published in:Nucleic acids research 2001-08, Vol.29 (16), p.3448
Main Authors: Onishi, Yoshiaki, Kiyama, Ryoiti
Format: Article
Language:English
Online Access:Get full text
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Summary:A class of curved DNA appears universally in eukaryotic genomic DNA at an average distance of ~680 bp and shows nucleosome positioning activity by having high affinity for histone core particles in an orientation- and position-dependent manner. Here, we report that the enhancer activity at DNase I hypersensitive site 2 (HS2) of the human [beta]-globin locus control region ([beta]-LCR) can be modulated by the curved DNA located at a distance of two nucleosomes from HS2 and that the nucleosome at the curved DNA regulates nearby nucleosome phases as a key nucleosome. Erythroid-specific nucleosome phases which caused deviation of the NF-E2 (p18-p45 dimer) binding site from the nucleosome dyad axis were over-represented when the distance between the key nucleosome and HS2 exceeded 80 bp longer than the original length. At this state, enhancer activity was ~50% of that in the original construct, presumably due to reduced binding of transcription factors.
ISSN:0305-1048
1362-4962