Flavodoxin overexpression reduces cadmium-induced damage in alfalfa root nodules

Flavodoxins are electron carrier flavoproteins that are involved in the response to oxidative stress in bacteria and cyanobacteria. Recently, we obtained Sinorhizobium meliloti bacteria that overexpressed a flavodoxin from the cyanobacterium Anabaena variabilis [Redondo et al. (2009) Plant Physiolog...

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Bibliographic Details
Published in:Plant and soil 2010, Vol.326 (1-2), p.109-121
Main Authors: Shvaleva, Alla, Coba de la Peña, Teodoro, Rincón, Ana, Morcillo, César N, García de la Torre, Vanesa S, Lucas, M. Mercedes, Pueyo, José J
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Alfalfa
Analysis
Animal, plant and microbial ecology
B lymphocytes
Bacteria
Bacteroids
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Cadmium
Crop science
Ecology
Economic plant physiology
Fundamental and applied biological sciences. Psychology
Gene expression
General agronomy. Plant production
Heavy metals
Life Sciences
Nitrogen fixation
Nodules
Oxidative stress
Physiological aspects
Plant biology
Plant pathology
Plant Physiology
Plant Sciences
Plants
Proteins
Regular Article
Soil and water pollution
Soil contamination
Soil pollution
Soil science
Soil Science & Conservation
Soil-plant relationships. Soil fertility
Soil-plant relationships. Soil fertility. Fertilization. Amendments
Starches
Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)
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Summary:Flavodoxins are electron carrier flavoproteins that are involved in the response to oxidative stress in bacteria and cyanobacteria. Recently, we obtained Sinorhizobium meliloti bacteria that overexpressed a flavodoxin from the cyanobacterium Anabaena variabilis [Redondo et al. (2009) Plant Physiology 149:1166-1178]. In the present work, tolerance to cadmium was evaluated in free-living transformed S. meliloti and in alfalfa plants nodulated by the flavodoxin-overexpressing rhizobia, in comparison with plants nodulated by wild-type bacteria. Overexpression of flavodoxin protected free-living S. meliloti from cadmium toxicity and had a positive effect on nitrogen fixation of alfalfa plants subjected to cadmium stress. Flavodoxin notably reduced cadmium-induced structural and ultrastructural alterations in alfalfa nodules. Putative protection mechanisms in flavodoxin-overexpressing nodules are discussed. Flavodoxin could have applications as a biotechnological tool to improve the symbiotic performance of alfalfa and other legumes in cadmium polluted soils.
ISSN:0032-079X
1573-5036
DOI:10.1007/s11104-009-9985-1