Hepatic esterase activity is increased in hepatocyte-like cells derived from human embryonic stem cells using a 3D culture system

Objectives The aim of the study is to generate a spherical three-dimensional (3D) aggregate of hepatocyte-like cells (HLCs) differentiated from human embryonic stem cells and to investigate the effect of the 3D environment on hepatic maturation and drug metabolism. Results Quantitative real-time PCR...

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Bibliographic Details
Published in:Biotechnology letters 2018-05, Vol.40 (5), p.755-763
Main Authors: Choi, Young-Jun, Kim, Hyemin, Kim, Ji-Woo, Yoon, Seokjoo, Park, Han-Jin
Format: Article
Language:English
Subjects:
Applied Microbiology
Bile ducts
Biochemistry
Biomedical and Life Sciences
Biotechnology
Butyrylcholinesterase - genetics
Carboxylic Ester Hydrolases - genetics
Cell culture
Cell Culture Techniques - methods
Cell Differentiation - drug effects
Cells, Cultured
Cholinesterase
Drug metabolism
Embryo cells
Embryos
Esterase
Esterases
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Enzymologic - drug effects
Hepatocytes - cytology
Hepatocytes - metabolism
Hepatotoxicity
Human Embryonic Stem Cells - cytology
Human Embryonic Stem Cells - metabolism
Humans
Life Sciences
Liver
Maturation
Metabolism
Microbiology
Original Research Paper
Oseltamivir
Oseltamivir - pharmacology
Secretion
Spheroids, Cellular - cytology
Spheroids, Cellular - metabolism
Stem cells
Up-Regulation - drug effects
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Summary:Objectives The aim of the study is to generate a spherical three-dimensional (3D) aggregate of hepatocyte-like cells (HLCs) differentiated from human embryonic stem cells and to investigate the effect of the 3D environment on hepatic maturation and drug metabolism. Results Quantitative real-time PCR analysis indicated that gene expression of mature hepatocyte markers, drug-metabolizing enzymes, and hepatic transporters was significantly higher in HLCs cultured in the 3D system than in those cultured in a two-dimensional system ( p  
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-018-2528-1