Anti-obesity effect of extract from fermented Curcuma longa L. through regulation of adipogenesis and lipolysis pathway in high-fat diet-induced obese rats

• Published in: Food & nutrition research 2016-01, Vol.60, p.1-9
• Main Authors: Kim, Ji Hye, Kim, Ok-Kyung, Yoon, Ho-Geun, Park, Jeongjin, You, Yanghee, Kim, Kyungmi, Lee, Yoo-Hyun, Choi, Kyung-Chul, Lee, Jeongmin, Jun, Woojin
• Format: Article
• Language: English
• Subjects: Acclimatization; Acetyl-CoA carboxylase; Adipocytes; Adipogenesis; Adiponectin; Adipose tissue; AMP; AMP-activated protein kinase; Aspergillus oryzae; Atherosclerosis; Body weight; Cholesterol; Curcuma longa; Diabetes; Diet; Dietary supplements; Enzymes; Ethanol; Fatty acids; Fatty-acid synthase; Fermented food; Flavor; Food industry; Fuel consumption; High cholesterol diet; High fat diet; Humidity; Industrial applications; Intubation; Kinases; Lipase; Lipids; Lipogenesis; Lipolysis; Lipoprotein lipase; Metabolism; mRNA; Obesity; Oxidation; Palatability; Proteins; Rats; Regulation; Rodents; Triglycerides; Weight control; Weight reduction
• ISSN: 1654-661X
• DOI: 10.3402/fnrv60.30428

Even though Curcuma longa L. possesses various biological activities, it has strong flavor and taste, which decrease consumer palatability and limit industrial applications in food. The present study investigates the effects of C. longa L. fermented with Aspergillus oryzae supplementation in 60% high-fat diet-induced obese rats measured by the activation of adipogenesis and lipolysis. Rats were divided into four groups (n =6 per group) after 1 week of acclimatization: a normal diet group comprised rats fed the AIN76A rodent diet; a high-fat diet-induced obese group with rats fed a 60% high-fat diet; a Garcinia cambogia treated group (positive control) with rats fed a 60% high-fat diet with G. cambogia 500 g/kg body weight (b.w.)/day; and an fermented C. longa L. 50% ethanolic extract treated group (FCE50) with rats fed a 60% high-fat diet with FCE50 500 g/kg b.w./day. Each group received the appropriate vehicle or sample daily by gastric intubation for 12 weeks. We found that FCE50 administration suppressed b.w. gain and reduced white adipose tissue weight, serum triglyceride (TG), and cholesterol in high-fat diet-induced obese rats. These results can be associated with the suppression of adipocyte differentiation and lipogenesis with a decrease in the mRNA expressions of fatty acid synthase, acetyl-CoA carboxylase, adipocyte protein 2, and lipoprotein lipase induced by FCE50 administration. In addition, FCE50 increased lipolysis and ß-oxidation by up-regulating the expression of lipases such as adipose triglyceride lipase, hormone-sensitive lipase, adiponectin, and AMP-activated protein kinase. These results suggest that FCE50 can be a candidate for the prevention of obesity via suppressing adipogenesis and promoting lipolysis.