Development of a New Amycolatopsis orientalis Strain for High-Yield Eremomycin Production

A multistep random UV mutagenesis of an eremomycin-producing strain Amycolatopsis orientalis VKPM Ac-1125 with rather low productivity (0.5 g/L) and the further selection of the most high-yield mutant strains have resulted in a highly productive E 13-26 strain. After optimization of the fermentation...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2017-12, Vol.53 (8), p.792-799
Main Authors: Dzhavakhiya, V. V., Glagoleva, E. V., Popova, E. D., Ovchinnikov, A. I., Skryabin, K. G., Shobolov, D. L., Balabanyan, V. Yu, Chernobrovkin, M. G.
Format: Article
Language:English
Subjects:
Antibiotics
Biochemistry
Biology
Biomedical and Life Sciences
Calcium chloride
Fermentation
Gene Engineering
Glycerol
Life Sciences
Meals
Medical Microbiology
Microbiology
Mutagenesis
Potassium phosphate
Potassium phosphates
Powder
Producers
Productivity
Selection
Skim milk
Soybeans
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Summary:A multistep random UV mutagenesis of an eremomycin-producing strain Amycolatopsis orientalis VKPM Ac-1125 with rather low productivity (0.5 g/L) and the further selection of the most high-yield mutant strains have resulted in a highly productive E 13-26 strain. After optimization of the fermentation medium by multifactor analysis, the most efficient composition for the fermentation medium was established, %: glycerol, 8; soybean meals, 2.0; MgCl 2 , 0.02; KH 2 PO 4 , 0.02; KNO 3 , 0.6; CaCl 2 , 0.3; skim milk powder, 0.46; and glucose, 0.8. The maximum productivity of this strain on the optimized medium reached 3.42 ± 0.17 g/L. Therefore, the obtained strain is considered to be very promising for further use in the selection and development of technology for the large-scale eremomycin production.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683817080026