Relevance of N-terminus amino acids in CCL28 mediated murine airway hyper-reactivity

Removing the first 3 amino acids (del3-CCL28) led to marked increase in potency, with significantly increased sRaw (p=0.001 versus unfolded del3-CCL28; n>=4) and reduced sGaw (p=0.011) at only the 0.3 μg/ml dose. The first 3 N terminal amino acids decrease potency of this effect by a log-fold, su...

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Bibliographic Details
Published in:Journal of allergy and clinical immunology 2018-02, Vol.141 (2), p.AB282-AB282
Main Authors: Rohlfing, Michelle, Santoro, Jennifer L., Hussain, Syed-Rehan A., Thomas, Monica, Volkman, Brian, Grayson, Mitchell H.
Format: Article
Language:English
Subjects:
Amino acids
Asthma
Chemokines
N-Terminus
Respiratory tract
Viral infections
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Summary:Removing the first 3 amino acids (del3-CCL28) led to marked increase in potency, with significantly increased sRaw (p=0.001 versus unfolded del3-CCL28; n>=4) and reduced sGaw (p=0.011) at only the 0.3 μg/ml dose. The first 3 N terminal amino acids decrease potency of this effect by a log-fold, suggesting these amino acids may interfere with the most efficient binding of CCL28 to its receptor.
ISSN:0091-6749
1097-6825
DOI:10.1016/j.jaci.2017.12.898