A Search for a New Cellular Model to Study the Pharmacological Activity of Progesterone Analogues

The possibility of using the endometrial cell line as a model for studying the pharmacological activity of progesterone analogues is considered. Conditions for obtaining and culturing of endometrial cell lines are described, the morphological characteristic is given, and the immunophenotypic profile...

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Bibliographic Details
Published in:Cell and tissue biology 2018, Vol.12 (1), p.57-65
Main Authors: Petrosyan, M. A., Melezhnikova, N. O., Domnina, A. P., Andryushina, V. A., Goryachaya, T. S., Petrova, L. I., Malysheva, O. V., Razygraev, A. V., Polyakova, V. O., Sapronov, N. S.
Format: Article
Language:English
Subjects:
17β-Estradiol
Biomedical and Life Sciences
Cell Biology
Cell lines
Endometrium
Estrogen receptors
Estrogens
Life Sciences
Physical characteristics
Progesterone
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Summary:The possibility of using the endometrial cell line as a model for studying the pharmacological activity of progesterone analogues is considered. Conditions for obtaining and culturing of endometrial cell lines are described, the morphological characteristic is given, and the immunophenotypic profile, karyotype, and expression of progesterone and estrogen receptors are presented. Not all studied endometrial lines showed the ability to decidualize cells under the action of hormonal inducers (combinations of estradiol with progesterone and its analogues). It appeared that lines sensitive to hormones are able to increase the secretion of specific markers of decidualization under the action of highly active gestagenes (progesterone analogues) to a greater extent than under action of progesterone. These data are a basis for further development of the cellular model for studying the pharmacological activity of gestagenic compounds.
ISSN:1990-519X
1990-5203
DOI:10.1134/S1990519X18010133