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A Search for a New Cellular Model to Study the Pharmacological Activity of Progesterone Analogues

The possibility of using the endometrial cell line as a model for studying the pharmacological activity of progesterone analogues is considered. Conditions for obtaining and culturing of endometrial cell lines are described, the morphological characteristic is given, and the immunophenotypic profile...

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Published in:	Cell and tissue biology 2018, Vol.12 (1), p.57-65
Main Authors:	Petrosyan, M. A., Melezhnikova, N. O., Domnina, A. P., Andryushina, V. A., Goryachaya, T. S., Petrova, L. I., Malysheva, O. V., Razygraev, A. V., Polyakova, V. O., Sapronov, N. S.
Format:	Article
Language:	English
Subjects:	17β-Estradiol Biomedical and Life Sciences Cell Biology Cell lines Endometrium Estrogen receptors Estrogens Life Sciences Physical characteristics Progesterone
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container_title	Cell and tissue biology
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creator	Petrosyan, M. A. Melezhnikova, N. O. Domnina, A. P. Andryushina, V. A. Goryachaya, T. S. Petrova, L. I. Malysheva, O. V. Razygraev, A. V. Polyakova, V. O. Sapronov, N. S.
description	The possibility of using the endometrial cell line as a model for studying the pharmacological activity of progesterone analogues is considered. Conditions for obtaining and culturing of endometrial cell lines are described, the morphological characteristic is given, and the immunophenotypic profile, karyotype, and expression of progesterone and estrogen receptors are presented. Not all studied endometrial lines showed the ability to decidualize cells under the action of hormonal inducers (combinations of estradiol with progesterone and its analogues). It appeared that lines sensitive to hormones are able to increase the secretion of specific markers of decidualization under the action of highly active gestagenes (progesterone analogues) to a greater extent than under action of progesterone. These data are a basis for further development of the cellular model for studying the pharmacological activity of gestagenic compounds.
doi_str_mv	10.1134/S1990519X18010133
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It appeared that lines sensitive to hormones are able to increase the secretion of specific markers of decidualization under the action of highly active gestagenes (progesterone analogues) to a greater extent than under action of progesterone. 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subjects	17β-Estradiol Biomedical and Life Sciences Cell Biology Cell lines Endometrium Estrogen receptors Estrogens Life Sciences Physical characteristics Progesterone
title	A Search for a New Cellular Model to Study the Pharmacological Activity of Progesterone Analogues
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