Copper toxicity and date palm (Phoenix dactylifera) seedling tolerance: Monitoring of related biomarkers

• Published in: Environmental toxicology and chemistry 2018-03, Vol.37 (3), p.797-806
• Main Authors: Chaâbene, Zayneb, Hakim, Imen Rekik, Rorat, Agnieszka, Elleuch, Amine, Mejdoub, Hafedh, Vandenbulcke, Franck
• Format: Article
• Language: English
• Subjects: Accumulation; Aminoacyltransferases - metabolism; Amylase activity; Amylases - metabolism; Antioxidants - metabolism; Antioxidative responses; Biomarkers; Biomarkers - metabolism; Biomarkers’ gene expression; Catalase; Copper; Copper - toxicity; Date palms; Elongation; Exposure; Gene expression; Gene Expression Regulation, Plant - drug effects; Genes, Plant; Germination; Germination - drug effects; Homeostasis; Hydrogen peroxide; Metallothionein; Metallothionein - metabolism; Oxidation-Reduction; Oxidizing agents; Palladium; Phoeniceae - chemistry; Phoenix dactylifera; Phytochelatin synthase; Plant stress; Proteins; Reference Standards; Reproducibility of Results; Seed germination; Seedlings; Seedlings - drug effects; Seedlings - physiology; Seeds; Seeds - drug effects; Seeds - growth & development; Seeds - metabolism; Software; Stress, Physiological - drug effects; Superoxide dismutase; Superoxide Dismutase - metabolism; Thiobarbituric acid; Toxicity
• ISSN: 0730-7268; 1552-8618
• DOI: 10.1002/etc.4007

Date palm (Phoenix dactylifera) seeds were exposed to different copper (Cu) solutions to examine plant stress responses. Low Cu concentrations (0.02 and 0.2 mM) caused an increase of seed germination, whereas higher Cu amounts (2 mM) significantly inhibited seed germination, delayed hypocotyl elongation, increased seedling mortality, and reduced the germination index by more than 90%. Metal‐related toxicity symptoms appeared after 15 d of 2 mM of Cu exposure. Biochemical activities such as amylase activity and redox balance elements were examined to study the relationship between external Cu amount and internal plant response. The present study showed that amylolytic activity was dose‐ and time‐dependent. Likewise, H2O2 production increased after exposure to Cu, which was correlated with thiobarbituric acid reactive substance (TBARS) accumulation. Furthermore at low Cu concentrations, superoxide dismutase (SOD) and catalase (CAT) activities increased, suggesting that date palm seed stimulated its metal homeostasis networks. However, the highest cupric ion amounts increased cell oxidant accumulation and reduced enzyme production. Gene expression level measures of P. dactylifera phytochelatin synthase (Pdpcs) and P. dactylifera metallothionein (Pdmt) encoding genes have been carried out to investigate the implication of PdPCS and PdMT proteins in Cu homeostasis and/or its sequestration. Phoenix dactylifera metallothionein induction reached a peak after 30 d of exposure to 0.2 mM of Cu. However, it was down‐regulated in plants exposed to higher Cu concentrations. In the same conditions, Pdpcs was overexpressed during 1 mo of exposure before it decreased thereafter. These observations provide a new insight into date palm cell response to Cu, a metal that can be toxic but that is also an essential element. Environ Toxicol Chem 2018;37:797–806. © 2017 SETAC