HPLC-DAD, ESI–MS/MS, and NMR of lycopene isolated from P. guajava L. and Its biotechnological applications

Psidium guajava L. have been reported to be a rich source of antioxidant compounds. Its carotenoids have been highlighted by their high antioxidant capacity, which offers several benefits for human health. In this sense, lycopene isomers need to be identified. In this work, the comprehensive chemica...

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Bibliographic Details
Published in:European journal of lipid science and technology 2018-03, Vol.120 (3), p.n/a
Main Authors: Amorim, Adriany G. N., Souza, Jessica M. T., Santos, Raimunda C., Gullón Estévez, Beatriz, Oliveira, Ana, Santos, Luiz F. A., Virgino, Adamor L. E., Mafud, Ana C., Petrilli, Helena, Mascarenhas, Yvonne, Delerue-Matos, Cristina, Pintado, Maria Manuela, Leite, José Roberto
Format: Article
Language:English
Subjects:
Antiinfectives and antibacterials
Antimicrobial activity
Antioxidants
bioactivity
Carotenoids
Cosmetics
E coli
ESI–MS/MS
Food industry
Guava
High-performance liquid chromatography
HPLC‐DAD
Ionization
Isomers
Liquid chromatography
Lycopene
NMR
Nuclear magnetic resonance
Pharmaceutical industry
Psidium guajava
Tomatoes
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Summary:Psidium guajava L. have been reported to be a rich source of antioxidant compounds. Its carotenoids have been highlighted by their high antioxidant capacity, which offers several benefits for human health. In this sense, lycopene isomers need to be identified. In this work, the comprehensive chemical characterization, by HPLC-DAD, MS/MS, and NMR, of lycopene isolated from P. guajava L., antioxidant and antimicrobial activity of lycopene extracts and isolated lycopene are evaluated. The FTIR results reported a structure with Z configuration, confirmed by UV-Vis, with λmax = 448, 473, and 505 nm for 5-Z lycopene. Furthermore, MS/MS positive ionization shows one fragment m/z 309 [M-227]+, relatively abundant for isolated lycopene. Experimental and Theoretical NMR studies revealed that guava may contain 5-Z lycopene because of the similarity found among the peaks. Lycopene extracts presented higher antioxidant activity than isolated lycopene, from both P. guajava L. and tomato, when measured by ABTS and ORAC (r2 = 0.9995 and r2 = 0.9992, respectively). In addition, lycopene extract shows antibacterial efficacy against E. coli, S. aureus, and L. innocua, presenting MBC values of 20 mg mL−1. These results suggest that lycopene extract have potential applicability for food, cosmetics, and pharmaceutical industry.
ISSN:1438-7697
1438-9312
DOI:10.1002/ejlt.201700330