Peculiarities of the Mass Spectrometric Detection of Anthocyanins in High-Performance Liquid Chromatography

The dependence of the degree of fragmentation of anthocyanin “molecular” ions on the cone voltage of a mass spectrometric electrospray ionization detector was studied. It was found that the voltage required for the fragmentation of 50% of original “molecular” ions, E f (0.5), increased with the numb...

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Bibliographic Details
Published in:Journal of analytical chemistry (New York, N.Y.) N.Y.), 2017-12, Vol.72 (14), p.1441-1445
Main Authors: Deineka, V. I., Sidorov, A. N., Chulkov, A. N., Deineka, L. A.
Format: Article
Language:English
Subjects:
Acetonitrile
Analytical Chemistry
Anthocyanins
Chemistry
Chemistry and Materials Science
Electric potential
Formic acid
Fragmentation
High performance liquid chromatography
Identification and classification
Ionization
Ionization counters
Ions
Mass spectrometry
Quadrupoles
Residues
Spectrometry
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Summary:The dependence of the degree of fragmentation of anthocyanin “molecular” ions on the cone voltage of a mass spectrometric electrospray ionization detector was studied. It was found that the voltage required for the fragmentation of 50% of original “molecular” ions, E f (0.5), increased with the number of glycoside residues. The fragmentation of glycosides proceeds with the removal of the entire residue regardless of their structures. In the case of 3,5-diglycosides, two types of fragment ions formed due to the loss of glycosidic residues from different positions; the ratio of their peak intensities is reciprocal to the ratio of the masses of residues eliminated. The values of E f (0.5) for monoglycosides (190 V), diglycosides (229 V), triglycosides (267 V), and for some acylated cyanidin-3,5-diglycosides are determined. These results were given for the gradient separation of anthocyanins by reversed-phase HPLC in aqueous acetonitrile containing 10 vol % of formic acid using quadrupole mass spectrometric detection.
ISSN:1061-9348
1608-3199
DOI:10.1134/S1061934817140040