A nile red-based near-infrared fluorescent probe for endogenous hydrogen polysulfides in living cells

Endogenous hydrogen polysulfides (H 2 S n ; n > 1) have attracted increasing attention because emerging studies have shown that it is actually the key signaling molecule rather than hydrogen sulfide (H 2 S). Therefore, developing probes for selective and sensitive detection of hydrogen polysulfid...

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Bibliographic Details
Published in:Analytical methods 2017-01, Vol.9 (45), p.6443-6447
Main Authors: Li, Kai-Bin, Chen, Feng-Zao, Zhang, Siqi, Shi, Wei, Han, De-Man, Cai, Chen, Chen, Cai-Xia
Format: Article
Language:English
Subjects:
Cells (biology)
Chemical synthesis
Esterification
Fluorescent indicators
Hydrogen
Hydrogen sulfide
I.R. radiation
Nitrobenzoic acid
Polysulfides
Signaling
Sulfide
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Summary:Endogenous hydrogen polysulfides (H 2 S n ; n > 1) have attracted increasing attention because emerging studies have shown that it is actually the key signaling molecule rather than hydrogen sulfide (H 2 S). Therefore, developing probes for selective and sensitive detection of hydrogen polysulfides is of great significance in biological science. In this study, a new fluorescent off–on probe for the selective and sensitive detection of hydrogen polysulfides (H 2 S n ) with near-infrared (NIR) fluorescent signal change has been designed and synthesized. The probe was synthesized by an esterification reaction coupling between a nile red dye and a bis-electrophilic H 2 S n capture group 2-fluoro-5-nitrobenzoic acid. The probe showed a specific off–on response to H 2 S n in aqueous solution with nanomolar LOD without interference by a range of competing sulfocompounds and several reactive nitrogen and oxygen species. Moreover, it can be used for imaging of endogenous H 2 S n in living cells.
ISSN:1759-9660
1759-9679
DOI:10.1039/C7AY02376F