Polyethyleneimine grafting and Cibacron Blue F3GA modifying poly(methylmethacrylate) magnetic microspheres for protein adsorption

BACKGROUND Polyethyleneimine (PEI) has attracted wide interest due to not only its good hydrophilicity but also its high density of amino groups across the polymer chain. In order to study in depth the effect of PEI as spacer arm on dye ligand modification and further protein adsorption, poly(methyl...

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Published in:Journal of chemical technology and biotechnology (1986) 2018-04, Vol.93 (4), p.994-1002
Main Authors: Dou, Yi‐Fang, Zhang, Dong‐Hao, Liu, Hang, Xia, Yu‐Pei, Zhi, Gao‐Ying
Format: Article
Language:English
Subjects:
Adsorption
affinity adsorption
Amino groups
Bovine serum albumin
bovine serum albumin (BSA)
Cibacron Blue F3GA
Dyes
Immobilization
Ionic strength
Ions
Ligands
magnetic microspheres
Microspheres
Polyethyleneimine
polyethyleneimine (PEI)
Polymethyl methacrylate
Polymethylmethacrylate
Protein adsorption
Proteins
Serum albumin
Temperature effects
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Summary:BACKGROUND Polyethyleneimine (PEI) has attracted wide interest due to not only its good hydrophilicity but also its high density of amino groups across the polymer chain. In order to study in depth the effect of PEI as spacer arm on dye ligand modification and further protein adsorption, poly(methylmethacrylate) (PMMA) magnetic microspheres were fabricated, grafted with PEI, and further modified with an affinity dye‐ligand, Cibacron Blue F3GA (CB). RESULTS CB density of these CB‐PEI‐PMMA microspheres was determined to be 370.7 µmol g‐1 for CB‐PEI 600‐PMMA, 355.2 µmol g‐1 for CB‐PEI 1800‐PMMA, and 317.6 µmol g‐1 for CB‐PEI 70000‐PMMA microspheres, respectively. Moreover, bovine serum albumin (BSA) adsorption capacity of PEI‐PMMA and CB‐PEI‐PMMA microspheres increased greatly compared with PMMA microspheres. The effects of temperature, BSA initial concentration, pH and ionic strength on BSA adsorption were studied. CB‐PEI 70000‐PMMA microspheres exhibited the maximum adsorption of 103.6 mg g‐1. CONCLUSION The results demonstrated the versatility of PEI as spacer arm. The information obtained from the present work could be utilized for rational design of carriers for protein adsorption or enzyme immobilization. © 2017 Society of Chemical Industry
ISSN:0268-2575
1097-4660
DOI:10.1002/jctb.5451