Expression of truncated forms of liver microsomal P450 cytochromes 2B4 and 2E1 in Escherichia coli: Influence of NH(2)-terminal region on localization in cytosol and membranes

In a study, variants of alcohol-inducible P450 2E1 as well as truncated forms of phenobarbital-inducible P450 2B4 were similarly expressed to determine the influence of the NH2-terminal region on the membrane-binding properties of Escherichia coli. The results indicated that alterations in the NH2-t...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1993-04, Vol.90 (7), p.2651
Main Authors: Pernecky, Steven J, Larson, Jane R, Philpot, Richard M, Coon, Minor J
Format: Article
Language:English
Subjects:
Bacteria
Liver
Membranes
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Summary:In a study, variants of alcohol-inducible P450 2E1 as well as truncated forms of phenobarbital-inducible P450 2B4 were similarly expressed to determine the influence of the NH2-terminal region on the membrane-binding properties of Escherichia coli. The results indicated that alterations in the NH2-terminal region can change the location of the cytochrome from largely membranous to largely cytosolic.
ISSN:0027-8424
1091-6490