Holistic protocol for callus culture optimization using statistical modelling

• Published in: Natural product research 2018-05, Vol.32 (9), p.1109-1117
• Main Authors: Fidemann, Tiago, de Araujo Pereira, Gabriela Aparecida, Bossard Nascimento, Lia, Moraes, Milena Cristina, Bertão, Mônica Rosa, da Silva, Regildo Márcio Gonçalves, Fernández Núñez, Eutimio Gustavo
• Format: Article
• Language: English
• Subjects: 2,4-D; Abiotic factors; Antioxidant activity; Antioxidants; Benzyladenine; bioprocess optimisation; Callus; Callus culture; Capsicum baccatum; Culture techniques; Dichlorophenoxyacetic acid; Factorial design; Flavonoids; Growth hormones; Illnesses; Mathematical models; Metabolites; Optimization; Oxidative stress; Peppers; Phenols; plant cell culture; polyphenolic compounds; Polyphenols; Secondary metabolites; statistical experimental designs; Statistical models
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2017.1380026

Plants endue a key role against illnesses caused by oxidative stress. These attributes are frequently associated with polyphenolic compounds. However, presence and concentration of secondary metabolites are affected by abiotic factors. The in vitro culture techniques can solve these drawbacks. Peppers can be a suitable alternative to obtain polyphenols. Aiming to optimise the callus culture stage from Capsicum baccatum to produce polyphenols, this work evaluated systemically the effects of the explant's origin (root, hypocotyl and cotyledon), growth hormone type (2,4-dichlorophenoxyacetic acid (2,4-D), benzylaminopurine (BAP) and a combination of 2,4-D/BAP at five-to-one ratio) and concentration (0.023-10.000 mg L −1 ) on callus culture efficiency parameters using a multilevel factorial design. The root explant in combination with BAP at 1.138 mg L − 1 ensured the optimal values of the assessed responses; ​callus mass (225.03 mg), antioxidant activity (35.95%), total phenols (11.48 mg of GAE/g DE) and flavonoids (15.92 mg of RU/g DE) production.